IFN-alpha/beta R1 Knockout HeLa Cell Lysate

Product Discontinued

IFN-alpha/beta R1 Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,-7 bp deletion in exon1 and Insertion of the selection cassette in exon1
• Gene: IFNAR1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for IFN-alpha/beta R1 Knockout HeLa Cell Lysate

• alpha-type antiviral protein
• AVP
• beta-type antiviral protein
• CRF2-1
• Cytokine receptor class-II member 1
• Cytokine receptor family 2 member 1
• human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC
• IFN-alpha/beta R1
• IFN-alpha/beta receptor 1
• IFN-alpha-REC
• IFNAR
• IFNAR1
• IFN-aR1
• IFNBR
• IFNbR1
• IFN-bR1
• IFN-R-1
• interferon (alpha, beta and omega) receptor 1
• interferon alpha/beta receptor 1
• interferon-alpha/beta receptor alpha chain
• interferon-beta receptor 1
• Type I interferon receptor 1

Background

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IFNAR1