IFN-alpha 2 Antibody (N27) - BSA Free

Western Blot: IFN-alpha 2 Antibody (N27) [NBP3-18803] - Western blot using IFN-alpha 2 antibody (N27)

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: N27
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.1 mg/ml

IFN-alpha 2 Antibody (N27) - BSA Free Summary

• Immunogen: E.coli derived recombinant human IFN-alpha 2c
• Epitope: 43aa-53aa, outside the immunodominant regions I and II
• Specificity: This antibody is specific for human IFN-alpha 2 and does not cross react with human IFN-alpha 1. It reacts with linear peptide 43aa-53aa, placing the epitope outside the immunodominant regions I and II.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFNA2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Frozen 1-2 ug/ml
  • Western Blot 1:100-1:2000
• Application Notes: N27 is one of many MoAbs described by Kontsek et al. (1991) directed against a site different from the two immunodominant sites called I and II. As such it is against an "atypical" site of the molecule. Peptide mapping for N27 has been done by Kontsek et al. Not recommended for IHC-paraffin sections.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for IFN-alpha 2 Antibody (N27) - BSA Free

• alpha-2a interferon
• Ifa2
• IFNA
• IFNA2
• IFNA2a
• IFNA2b
• IFNA2c
• IFNAA
• IFNalpha 2
• IFN-alpha 2
• IFN-alpha-2
• IFN-alphaA
• INFA2
• interferon alpha 2b
• interferon alpha A
• interferon alpha-2
• Interferon alpha-A
• interferon, alpha 2
• LeIF A
• MGC125764
• MGC125765

Background

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of twoenzymes: a protein kinase and an oligoadenylate synthetase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFNA2