IFITM1 Antibody - BSA Free

Western Blot: IFITM1 Antibody [NBP1-77171] - 3T3 cell lysate with IFITM1 antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry/Immunofluorescence: IFITM1 Antibody [NBP1-77171] - Jurkat cells with IFITM1 antibody at 20 ug/mL.

Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody - BSA Free [NBP1-77171] - Immunofluorescence of IFITM1 in Jurkat cells with IFITM1 antibody at 20 ug/mL.

ADAR2 deficiency leads to upregulation of type 1 IFN response. (A) Basal expression of ISGs—RIG‐I and IFITM1 in Mero95 and RN5 WT, KD and rescue cells. Protein levels are represented relative to the respective WT. Protein size is represented in kDa. n = 3. One‐way ANOVA with post‐hoc Tukey's test. Error bars indicate SEM. (B) Expression of ISGs—Rig‐I and Ifitm1 upon silencing of Mavs in RN5 ADAR2 KD cells. Protein levels are represented relative to siNT. Protein size is represented in kDa. n = 3. Paired t‐test. Error bars indicate SEM. (C) Expression of ISGs – RIG‐I and IFITM1 upon silencing of STING in Mero95 ADAR2 KD cells. Protein levels are represented relative to siNT. Protein size is represented in kDa. n = 3. Paired t‐test. Error bars indicate SEM. (D) Representative images of senescence‐associated beta ‐Gal staining in Mero95 WT, KO and rescue cells (left). Quantification of beta ‐Gal positive cells (right). n = 3 (with at least 15 images from each group). One‐way ANOVA with post‐hoc Tukey's test. Error bars indicate SEM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36221913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

ADAR2 deficiency leads to upregulation of type 1 IFN response. (A) Basal expression of ISGs—RIG‐I and IFITM1 in Mero95 and RN5 WT, KD and rescue cells. Protein levels are represented relative to the respective WT. Protein size is represented in kDa. n = 3. One‐way ANOVA with post‐hoc Tukey's test. Error bars indicate SEM. (B) Expression of ISGs—Rig‐I and Ifitm1 upon silencing of Mavs in RN5 ADAR2 KD cells. Protein levels are represented relative to siNT. Protein size is represented in kDa. n = 3. Paired t‐test. Error bars indicate SEM. (C) Expression of ISGs – RIG‐I and IFITM1 upon silencing of STING in Mero95 ADAR2 KD cells. Protein levels are represented relative to siNT. Protein size is represented in kDa. n = 3. Paired t‐test. Error bars indicate SEM. (D) Representative images of senescence‐associated beta ‐Gal staining in Mero95 WT, KO and rescue cells (left). Quantification of beta ‐Gal positive cells (right). n = 3 (with at least 15 images from each group). One‐way ANOVA with post‐hoc Tukey's test. Error bars indicate SEM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36221913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IFITM1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide near the center of human IFITM1. The immunogen is located within amino acids 40 - 90 of IFITM1. Amino Acid Squence: CAFAYSVKSRDRKMVGD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IFITM1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IFITM1 Peptide (NBP1-77171PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IFITM1 Antibody - BSA Free

• CD225 antigen
• CD225
• CD225Leu-13 antigen
• IFI17
• IFI17LEU13,9-27
• IFITM1
• interferon induced transmembrane protein 1 (9-27)
• Interferon-induced protein 17
• interferon-induced transmembrane protein 1
• Interferon-inducible protein 9-27
• Leu-13

Background

IFITM1 (Interferon inducible transmembrane protein 1) is a member of the IFN-inducible transmembrane protein family. It is an essential mediator of interferon-gamma-induced antiproliferation and plays a role in the control of cell growth. IFITM1 is upregulated in several tumor types and may be useful as a tumor biomarker. Both mouse IFITM1 and IFITM3 are expressed on the cell surfaces of primordial germ cells in a developmentally-regulated manner. IFITM1 activity is required for primordial germ cell transit, and IFITM1 acts as a repulsive molecule by repelling non-IFITM1-expressing primordial germ cells from the mesoderm into the endoderm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFITM1 Antibody (NBP1-77171)(2)

Publications using NBP1-77171

• Hariharan A, Qi W, Rehrauer H et al. Heterogeneous RNA editing and influence of ADAR2 on mesothelioma chemoresistance and the tumor microenvironment Molecular Oncology 2022-12-01 [PMID: 36221913]
• Sun S, Frontini F, Qi W, Hariharan A et Al. Endogenous retrovirus expression activates type-I interferon signaling in an experimental mouse model of mesothelioma development Cancer Lett 2021-03-13 [PMID: 33713739]

Bioinformatics

• Gene Symbol: IFITM1
• Uniprot:
  • NP_003632()