ICAM-4 Overexpression Lysate

Western Blot: ICAM4 Overexpression Lysate (Adult Normal) [NBL1-11808] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM4.

• Reactivity: Hu
• Applications: WB

ICAM-4 Overexpression Lysate Summary

• Description:

  ICAM-4 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC200636
  
  Accession#: NM_001039132
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: ICAM4

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 27.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for ICAM-4 Overexpression Lysate

• CD242 antigen
• CD242
• ICAM4
• ICAM-4
• intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
• intercellular adhesion molecule 4 (LW blood group)
• intercellular adhesion molecule 4
• intercellular adhesion molecule 4, Landsteiner-Wiener blood group
• Landsteiner-Wiener blood group antigen a
• Landsteiner-Wiener blood group glycoprotein
• Landsteiner-Wiener blood group
• LWLW blood group protein

Background

This gene encodes the Landsteiner-Wiener (LW) blood group antigen(s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. This ICAM protein contains 2 Ig-like C2-type domains and binds to the leukocyte adhesion LFA-1 protein. The molecular basis of the LW(A)/LW(B) blood group antigens is a single aa variation at position 100; Gln-100=LW(A) and Arg-100=LW(B). Alternative splicing results in multiple transcript variants encoding distinct isoforms. Transcript Variant: Compared to variant 1, this variant (3) uses an alternate acceptor splice site at exon 2 which results in a frameshift and translation termination further downstream. The encoded isoform (3) thus has the same N-terminus, but has alterations to the C-terminal second Ig-like C2-type domain, the transmembrane domain, and the cytoplasmic domain, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ICAM4