Human ICAM-2/CD102 ELISA Kit (Colorimetric)

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ELISA: Human ICAM-2/CD102 ELISA Kit (Colorimetric) [NBP1-91181] - man ICAM-2/CD102 ELISA Kit [NBP1-91181] - Data for Human sICAM-2 Platinum ELISA

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), amniotic fluid, urine, whole blood
Standard Curve Range
0.75 - 24.0 U/mL
Sensitivity
0.30 U/mL

Order Details

Human ICAM-2/CD102 ELISA Kit (Colorimetric) Summary

Specificity
This antibody targets the soluble protein. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into ICAM-2 positive serum. There was no crossreactivity detected, notably not with ICAM-1 and ICAM-3.
Standard Curve Range
0.75 - 24.0 U/mL
Sensitivity
0.30 U/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 7.00%
Intra-Assay
CV% < 6.70%
Sample Volume
100 uL (1:20 prediluted)
Kit Type
ELISA Kit (Colorimetric)
Gene
ICAM2

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-2
  3. Biotin Conjugate Diluent
  4. Biotin-Conjugate anti-human sICAM-2 monoclonal antibody
  5. Human sICAM-2 Standard lyophilized, 24.00 U/ml upon reconstitution
  6. Standard Buffer Diluent (10x)
  7. Stop Solution (1.8N H2SO4)
  8. Streptavidin-HRP Diluent
  9. Streptavidin-HRP-Concentrate
  10. Substrate Solution (tetramethyl-benzidine and buffered hydrogen peroxide)
  11. Wash Buffer Concentrate 200x

Alternate Names for Human ICAM-2/CD102 ELISA Kit (Colorimetric)

  • CD102 antigen
  • CD102
  • ICAM2
  • ICAM-2
  • intercellular adhesion molecule 2

Background

Intercellular Adhesion Molecule-2 (ICAM-2) is a 60 kDa surface glycoprotein that promotes adhesion between immune cells and the vascular endothelium. ICAM-2 is a member of the Ig-superfamily with two immunoglobulin-like extracellular domains. ICAM-2 is much more closely related to the two N-terminal domains of ICAM-1 (34% identity) than either ICAM-1 or ICAM-2 is to other members of the Ig-superfamily, demonstrating the existence of a subfamily of Ig-like ligands that bind the same integrin receptor. ICAM-2 is broadly distributed on hematopoietic cells. On resting lymphocytes, the ICAM-2 expression is several-fold higher than that seen with ICAM-1, while monocytes express equivalent levels of ICAM-1 and ICAM-2. In contrast to ICAM-1, neutrophils stain negative for ICAM-2. Just little or no ICAM-2 staining can be demonstrated on other cell lines, the sole exception being resting vascular endothelial cells, which possess high levels of ICAM-2, and small clusters of cells in lymphoid tissue germinal centres. ICAM-2 expression is unaffected by a variety of inflammatory cytokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ICAM2