Human ICAM-2/CD102 ELISA Kit (Colorimetric)

ELISA: Human ICAM-2/CD102 ELISA Kit (Colorimetric) [NBP1-91181] - man ICAM-2/CD102 ELISA Kit [NBP1-91181] - Data for Human sICAM-2 Platinum ELISA

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), amniotic fluid, urine, whole blood
• Standard Curve Range: 0.75 - 24.0 U/mL
• Sensitivity: 0.30 U/mL

Human ICAM-2/CD102 ELISA Kit (Colorimetric) Summary

• Specificity: This antibody targets the soluble protein. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into ICAM-2 positive serum. There was no crossreactivity detected, notably not with ICAM-1 and ICAM-3.
• Standard Curve Range: 0.75 - 24.0 U/mL
• Sensitivity: 0.30 U/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.00%
• Intra-Assay: CV% < 6.70%
• Sample Volume: 100 uL (1:20 prediluted)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ICAM2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human ICAM-2/CD102 ELISA Kit (Colorimetric)

• CD102 antigen
• CD102
• ICAM2
• ICAM-2
• intercellular adhesion molecule 2

Background

Intercellular Adhesion Molecule-2 (ICAM-2) is a 60 kDa surface glycoprotein that promotes adhesion between immune cells and the vascular endothelium. ICAM-2 is a member of the Ig-superfamily with two immunoglobulin-like extracellular domains. ICAM-2 is much more closely related to the two N-terminal domains of ICAM-1 (34% identity) than either ICAM-1 or ICAM-2 is to other members of the Ig-superfamily, demonstrating the existence of a subfamily of Ig-like ligands that bind the same integrin receptor. ICAM-2 is broadly distributed on hematopoietic cells. On resting lymphocytes, the ICAM-2 expression is several-fold higher than that seen with ICAM-1, while monocytes express equivalent levels of ICAM-1 and ICAM-2. In contrast to ICAM-1, neutrophils stain negative for ICAM-2. Just little or no ICAM-2 staining can be demonstrated on other cell lines, the sole exception being resting vascular endothelial cells, which possess high levels of ICAM-2, and small clusters of cells in lymphoid tissue germinal centres. ICAM-2 expression is unaffected by a variety of inflammatory cytokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ICAM2