Human ICAM-2/CD102 ELISA Kit (Colorimetric) Summary
Specificity |
This antibody targets the soluble protein. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into ICAM-2 positive serum. There was no crossreactivity detected, notably not with ICAM-1 and ICAM-3. |
Standard Curve Range |
0.75 - 24.0 U/mL |
Sensitivity |
0.30 U/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 7.00% |
Intra-Assay |
CV% < 6.70% |
Sample Volume |
100 uL (1:20 prediluted) |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
ICAM2 |
Applications/Dilutions
Dilutions |
|
Application Notes |
This kit is an enzyme-linked immunosorbent assay for quantitative detection. |
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-2
- Biotin Conjugate Diluent
- Biotin-Conjugate anti-human sICAM-2 monoclonal antibody
- Human sICAM-2 Standard lyophilized, 24.00 U/ml upon reconstitution
- Standard Buffer Diluent (10x)
- Stop Solution (1.8N H2SO4)
- Streptavidin-HRP Diluent
- Streptavidin-HRP-Concentrate
- Substrate Solution (tetramethyl-benzidine and buffered hydrogen peroxide)
- Wash Buffer Concentrate 200x
|
Alternate Names for Human ICAM-2/CD102 ELISA Kit (Colorimetric)
Background
Intercellular Adhesion Molecule-2 (ICAM-2) is a 60 kDa surface glycoprotein that promotes adhesion between immune cells and the vascular endothelium. ICAM-2 is a member of the Ig-superfamily with two immunoglobulin-like extracellular domains. ICAM-2 is much more closely related to the two N-terminal domains of ICAM-1 (34% identity) than either ICAM-1 or ICAM-2 is to other members of the Ig-superfamily, demonstrating the existence of a subfamily of Ig-like ligands that bind the same integrin receptor. ICAM-2 is broadly distributed on hematopoietic cells. On resting lymphocytes, the ICAM-2 expression is several-fold higher than that seen with ICAM-1, while monocytes express equivalent levels of ICAM-1 and ICAM-2. In contrast to ICAM-1, neutrophils stain negative for ICAM-2. Just little or no ICAM-2 staining can be demonstrated on other cell lines, the sole exception being resting vascular endothelial cells, which possess high levels of ICAM-2, and small clusters of cells in lymphoid tissue germinal centres. ICAM-2 expression is unaffected by a variety of inflammatory cytokines.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for ICAM-2/CD102 ELISA Kit (NBP1-91181). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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