![Immunohistochemistry-Paraffin: HYI Antibody (2A2) [H00081888-M01] - Analysis of monoclonal antibody to HYI on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/HYI-Antibody-2A2-Immunohistochemistry-Paraffin-H00081888-M01-img0004.jpg "Immunohistochemistry-Paraffin: HYI Antibody (2A2) [H00081888-M01] - Analysis of monoclonal antibody to HYI on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | 2A2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Mouse HYI Antibody (2A2) - Azide and BSA Free (H00081888-M01) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | HYI (AAH06140, 1 a.a. ~ 217 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGLGAVPGRQAAFREGLEQAVRYAKALGCPRIHLMAGRVPQGADRIAVKAEMEAVFLENLRHAAGVLAQEDLVGLLEPINTRITDPQYFLDTPQQAAAILQKVGRPNLQLQMDIFHWQIMDGNLTGNIREFLPIVGHVQVAQVPGRGEPSSPGELNFPYLFQLLENEGYKGFVGCEYQPRGDTVEGLSWLRSYWDRRATQRLASEGPHTTHVPPDSE |
| Specificity | HYI - hydroxypyruvate isomerase homolog (E. coli) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HYI |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HYI Antibody (H00081888-M01)Find related products by research area.
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![Sandwich ELISA: HYI Antibody (2A2) [H00081888-M01] - Detection limit for recombinant GST tagged HYI is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HYI-Antibody-2A2-Sandwich-ELISA-H00081888-M01-img0005.jpg "Sandwich ELISA: HYI Antibody (2A2) [H00081888-M01] - Detection limit for recombinant GST tagged HYI is approximately 0.1ng/ml as a capture antibody.")
![Western Blot ID1 Antibody [Unconjugated]](https://images.novusbio.com/images2/ID1_AF4377_Western_Blot_5453.jpg)
![Immunocytochemistry ID1 Antibody [Unconjugated]](https://images.novusbio.com/images2/ID1_AF4377_Immunocytochemistry_12849.jpg)
![Simple Western ID1 Antibody [Unconjugated]](https://images.novusbio.com/images2/af4377_human-mouse-id1-affinity-purified-polyclonal-ab-simple-western-482021152036.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")