HYAL2 Overexpression Lysate (Native)


Western Blot: HYAL2 Overexpression Lysate (Adult Normal) [NBL1-11794] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HYAL2.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

HYAL2 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC200552 and based on accession number NM_033158. The protein contains a C-terminal DDK tag.
Homo sapiens hyaluronoglucosaminidase 2 (HYAL2), transcript variant 2, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
53.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for HYAL2

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HYAL2 Overexpression Lysate (Native)

  • EC
  • Hyal-2
  • hyaluronidase 2
  • hyaluronoglucosaminidase 2
  • Hyaluronoglucosaminidase-2
  • LuCa-2
  • LUCA2hyaluronidase-2
  • Lung carcinoma protein 2
  • lysosomal hyaluronidase
  • PH20 homolog
  • PH-20 homolog


This gene encodes a protein which is similar in structure to hyaluronidases. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. Varying functions have been described for this protein. It has been described as a lysosomal hyaluronidase which is active at a pH below 4 and specifically hydrolyzes high molecular weight hyaluronan. It has also been described as a GPI-anchored cell surface protein which does not display hyaluronidase activity but does serve as a receptor for the oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes two alternatively spliced transcript variants which differ only in the 5' UTR. [provided by RefSeq]. Transcript Variant: This variant (2) uses an alternate first exon in the 5' UTR. It encodes the same protein as transcript variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for HYAL2 Lysate (NBL1-11794) (0)

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Video Protocols

WB Video Protocol

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Contact Information

Product PDFs


Gene Symbol HYAL2

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