HVEM/TNFRSF14 Antibody

Product Discontinued

HVEM/TNFRSF14 Antibody Summary

• Immunogen: Peptide with sequence C-SVQRKRQEAEGE corresponding to internal region according to NP_003811.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFRSF14
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 30 kDa band observed in human thymus and human tonsil lysates (calculated MW of 30.4 kDa band according to NP_003811.2).
  IHC: Paraffin embedded Human Kidney.
• Control:

  Human Thymus Whole Tissue Lysate (Adult Whole Normal)
  HVEM/TNFRSF14 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HVEM/TNFRSF14 Antibody

• ATAR
• CD270 antigen
• CD270
• CD40-like protein
• Herpes virus entry mediator A
• Herpesvirus entry mediator A
• HveA
• HVEM
• HVEMTR2HVEAATAR
• LIGHTR
• TNFRSF14
• tumor necrosis factor receptor superfamily member 14
• tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator)
• Tumor necrosis factor receptor-like 2
• tumor necrosis factor receptor-like gene2

Background

HVEM (herpes virus entry mediator), TR2 (TNF receptor like molecule) and ATAR (another TRAF-associated receptor), is a type I membrane protein belonging to the TNF receptor superfamily. This receptor mediates herpes virus entry into cells during infection. TNFRSF14 is able to inhibit the proliferation, activation, and cytokine production of T cells. It has an extracellular domain containing several cysteine-rich repeats and a short cytoplasmic region containing a TRAF (TNF receptor-associated factor) interaction domain. The extracellular domain of TNFRSF14 interacts with the herpes simplex virus envelope glycoprotein D. TNFRSF14 binds two cellular ligands: lymphotoxin alpha and LIGHT. LIGHT is a transmembrane protein expressed and shed from the surface of activated T cells, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes. The LIGHT:TNFRSF14 interaction controls immune response functions by cell death induction as well as cell activation. TNFRSF14 is expressed by peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HVEM/TNFRSF14 Antibody (NB100-2433)(1)

Publication using NB100-2433

• Cheung TC, Humphreys IR, Potter KG et al. From The Cover: Evolutionarily divergent herpesviruses modulate T cell activation by targeting the herpesvirus entry mediator cosignaling pathway. Proc Natl Acad Sci U S A 2005-09-13 [PMID: 16131544]

Bioinformatics

• Gene Symbol: TNFRSF14
• Entrez:
  • 8764(Human)
• Uniprot:
  • NP_003811.2(Human)