Recombinant Human HuR/ELAVL1 GST (N-Term) Protein

SDS-Page: Recombinant Human HuR/ELAVL1 Protein [H00001994-P01]

• Reactivity: Hu
• Applications: WB, ELISA, MA, PAGE, AP

Recombinant Human HuR/ELAVL1 GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-326 of Human ELAVL1
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: ELAVL1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • SDS-Page
  • Western Blot
• Theoretical MW: 62.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human HuR/ELAVL1 GST (N-Term) Protein

• ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• ELAV-like protein 1
• embryonic lethal, abnormal vision, drosophila, homolog-like 1
• Hu antigen R
• Hua
• Hu-antigen R
• HuR
• HURELAV1
• MelG

Background

The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for HuR/ELAVL1 Recombinant Protein (H00001994-P01)(3)

Publications using H00001994-P01

• Yurdagul A, Subramanian M, Wang X et al. Macrophage Metabolism of Apoptotic Cell-Derived Arginine Promotes Continual Efferocytosis and Resolution of Injury Cell Metab. 2020-01-17 [PMID: 32004476]
• Hans CP, Sharma N, Sen S et al. Transcriptomics Analysis Reveals New Insights into the Roles of Notch1 Signaling on Macrophage Polarization Sci Rep 2019-05-29 [PMID: 31142802]
• Herjan T, Yao P, Qian W et al. HuR Is Required for IL-17-Induced Act1-Mediated CXCL1 and CXCL5 mRNA Stabilization. J Immunol 2013-07-15 [PMID: 23772036]

Bioinformatics

• Gene Symbol: ELAVL1