Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit Summary

Background
The Quantikine Human PCSK9 immunoassay is a 4.5 hour solid phase ELISA designed to measure PCSK9 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human PCSK9 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural PCSK9 showed linear curves that were parallel to the standard curves obtained using the... Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring PCSK9.
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Specificity
Natural and recombinant human PCSK9. This assay recognizes free and LDLR-bound PCSK9.
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
PCSK9

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DPC900.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit

  • EC 3.4.21
  • EC 3.4.21.111
  • FH3
  • FH3neural apoptosis regulated convertase 1
  • FHCL3
  • HCHOLA3
  • hypercholesterolemia, autosomal dominant 3
  • LDLCQ1
  • NARC1
  • NARC-1
  • NARC-1convertase subtilisin/kexin type 9 preproprotein
  • NARC1EC 3.4.21.-
  • Neural apoptosis-regulated convertase 1
  • PC9
  • PCSK9
  • Proprotein Convertase 9
  • proprotein convertase subtilisin/kexin type 9
  • Subtilisin/kexin-like protease PC9

Background

Proprotein convertase subtilisin kexin 9 (PCSK9), also named neural apoptosis-regulated convertase 1 (NARC-1), is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. The full-length protein has 692 amino acids, including a signal peptide, a pro- domain, and a catalytic domain. PCSK9 is highly expressed in the liver, intestine, and kidney. It is initially synthesized as a soluble 74 kDa precursor protein. In the endoplasmic reticulum, it undergoes autocatalytic intramolecular cleavage to generate a 14 kDa pro- domain and a 60 kDa catalytic domain. These two domains remain associated when PCSK9 is secreted outside the cells (1-3). The primary physiologic function of PCSK9 is to mediate the degradation of low density lipoprotein receptor (LDL R). Early observations indicated that gain-of-function missense mutations in the PCSK9 gene can cause an autosomal dominant form of hypercholesterolemia (4, 5). The expression of PCSK9 was observed to be up-regulated by the sterol regulatory element binding proteins (SREBPs), a family of transcription factors that are responsible for the upregulation of genes involved in cholesterol and fatty acid metabolism, such as the LDL R gene (6, 7). Further experimental evidence revealed that in mice, when the PCSK9 gene was knocked out, the number of LDL R in hepatocytes increased, whereas when PCSK9 was over-expressed, the amount of LDL R protein was reduced in the liver (8, 9). In humans, genetic analyses have shown that individuals who have nonsense or loss-of-function mutations in the PCSK9 gene have significantly lower plasma LDL cholesterol levels (10, 11). These investigations clearly indicated that PCSK9 plays a key role in reducing the hepatic LDL R levels. Recently, the underlying mechanism has been uncovered: under normal physiologic conditions, the LDL R is internalized on the cell surface and directed to the endosomes in order to be recycled back to the cell surface. PCSK9 binds to the EGF domain of the LDL R and prevents LDL R from being sorted to the endosomes. Instead, the PCSK9/LDL R complex is redistributed to the lysosomes for degradation (12-14). As such, PCSK9 regulates the amount of LDL R in the circulation and modulates cholesterol levels. Serum PCSK9 concentrations have been found to be directly associated with cholesterol levels (15, 16). Since individuals with loss-of-function PCSK9 mutations have strikingly reduced risk of coronary heart diseases, PCSK9 has become an attractive drug target in recent years (17, 18). One approach is to generate small molecules that are able to interfere with PCSK9 autoactivation and its interaction with LDL R. Other approaches aiming to reduce the amounts of PCSK9 in the circulation, such as small interfering RNAs (siRNAs), have also shown promise (19, 20).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Proprotein Convertase 9/PCSK9 (DPC900)(22)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Porcine, Transgenic Mouse.


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Human
(17)
Mouse
(2)
Rat
(1)
Porcine
(1)
Transgenic Mouse
(1)
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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using DPC900 Applications Species
CJ Cui, JL Jin, LN Guo, J Sun, NQ Wu, YL Guo, G Liu, Q Dong, JJ Li Beneficial impact of epigallocatechingallate on LDL-C through PCSK9/LDLR pathway by blocking HNF1&alpha and activating FoxO3a J Transl Med, 2020;18(1):195. 2020 [PMID: 32398139] (Human) Human
JA Krahel, A Baran, TW Kami?ski, M Maciaszek, I Flisiak Methotrexate Decreases the Level of PCSK9-A Novel Indicator of the Risk of Proatherogenic Lipid Profile in Psoriasis. The Preliminary Data J Clin Med, 2020;9(4):. 2020 [PMID: 32225075] (Human) Human
YX Cao, JL Jin, D Sun, HH Liu, YL Guo, NQ Wu, RX Xu, CG Zhu, Q Dong, J Sun, JJ Li Circulating PCSK9 and cardiovascular events in FH patients with standard lipid-lowering therapy J Transl Med, 2019;17(1):367. 2019 [PMID: 31711505] (Human) Human
C Li, Z Hu, W Zhang, J Yu, Y Yang, Z Xu, H Luo, X Liu, Y Liu, C Chen, Y Cai, X Xia, X Zhang, DZ Wang, G Wu, C Zeng Regulation of Cholesterol Homeostasis by a Novel Long Non-coding RNA LASER Sci Rep, 2019;9(1):7693. 2019 [PMID: 31118464] (Human) Human
A Carreras, LS Pane, R Nitsch, K Madeyski-B, M Porritt, P Akcakaya, A Taheri-Gha, E Ericson, M Bjursell, M Perez-Alca, F Seeliger, M Althage, R Knöll, R Hicks, LM Mayr, R Perkins, D Lindén, J Borén, M Bohlooly-Y, M Maresca In vivo genome and base editing of a human PCSK9 knock-in hypercholesterolemic mouse model BMC Biol., 2019;17(1):4. 2019 [PMID: 30646909] (Transgenic Mouse) Transgenic Mouse
KR Genga, C Lo, MS Cirstea, FS Leitao Fil, KR Walley, JA Russell, A Linder, GA Francis, JH Boyd Impact of PCSK9 loss-of-function genotype on 1-year mortality and recurrent infection in sepsis survivors EBioMedicine, 2018;0(0):. 2018 [PMID: 30473376] (Human) Human
P Akcakaya, ML Bobbin, JA Guo, J Malagon-Lo, K Clement, SP Garcia, MD Fellows, MJ Porritt, MA Firth, A Carreras, T Baccega, F Seeliger, M Bjursell, SQ Tsai, NT Nguyen, R Nitsch, LM Mayr, L Pinello, M Bohlooly-Y, MJ Aryee, M Maresca, JK Joung In vivo CRISPR editing with no detectable genome-wide off-target mutations Nature, 2018;0(0):. 2018 [PMID: 30209390] (Mouse) Mouse
C Vlachopoul, I Koutagiar, D Terentes-P, I Skoumas, A Rigatou, A Miliou, AN Skliros, S Pantou, K Filis, D Tousoulis Relationship of PCSK9 levels with indices of vascular function and subclinical atherosclerosis in patients with familial dyslipidaemias Hellenic J Cardiol, 2018;0(0):. 2018 [PMID: 29807195] (Human) Human
P Lebeau, K Platko, AA Al-Hashimi, JH Byun, Š Lhoták, N Holzapfel, G Gyulay, SA Igdoura, D Cool, B Trigatti, NG Seidah, RC Austin Loss-of-function PCSK9 mutants evade the unfolded protein response sensor, GRP78, and fail to induce endoplasmic reticulum stress when retained J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29593095] (Human) Human
F Yuan, L Guo, KH Park, JR Woollard, K Taek-Geun, K Jiang, T Melkamu, B Zang, SL Smith, SC Fahrenkrug, FD Kolodgie, A Lerman, R Virmani, LO Lerman, DF Carlson Ossabaw Pigs With a PCSK9 Gain-of-Function Mutation Develop Accelerated Coronary Atherosclerotic Lesions: A Novel Model for Preclinical Studies J Am Heart Assoc, 2018;7(6):. 2018 [PMID: 29572319] (Porcine) Porcine
Show All 22 Publications.

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Product General Protocols

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FAQs for Proprotein Convertase 9/PCSK9 (DPC900). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP DPC900
HRP DPC900
HRP DPC900

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Bioinformatics

Gene Symbol PCSK9