Human Phospho-Histone H2AX (S139) DuoSet IC ELISA, 2 Plate

A172 human glioblastoma cells were treated with Gas-6-containing conditioned media1 from the human lung cell line, WI38, for fifteen minutes to induce tyrosine phosphorylation of Axl. Lysates were serially diluted and analyzed by (A) IP-Western blot and (B) this DuoSet IC ELISA. IPs were done using an Anti-Human Axl Monoclonal Antibody and Anti-Mouse IgG Agarose. Immunoblots were incubated with a Biotinylated Anti-Human Phospho-tyrosine Monoclonal Antibody (Catalog # BAM1676) to detect phospho-Axl. Bands were visualized with Streptavidin-HRP (Catalog # DY998) followed by chemiluminescent detection.

A172 cells were untreated or treated with Gas-6-containing conditioned media1 from the human lung cell line, WI38, for 15 minutes. ELISA and IP-Western blot (inset) analyses were done using 100 µg and 400 µg of lysate, respectively. IP-Western blots for phospho-Axl (p-Axl) were done as described in Figure 1. Blots were stripped and total Axl (Axl) was detected using a Biotinylated Anti-Human Axl Polyclonal Antibody (Catalog # BAF154).

A172 cells were treated with Gas-6-containing conditioned media1 from the human lung cell line, WI38, for 15 minutes. The indicated amounts of recombinant extracellular domains of human Axl/Fc Chimera (Catalog # 154-AL), human Dtk/Fc Chimera (Catalog # 859-DK) or human TrkA/Fc Chimera (Catalog # 175-TK) were added to 125 µg of lysate and analyzed using this DuoSet IC ELISA. Competition was observed only with recombinant human Axl.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Phospho-Histone H2AX (S139) DuoSet IC ELISA, 2 Plate Summary

• Modification: p Ser139
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: H2AX

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Phospho-Histone H2AX (S139) DuoSet IC ELISA, 2 Plate

• H2A.X
• H2A/X
• H2AFX
• Histone H2AX

Background

Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.

Publications for Histone H2AX (DYC2288-2)(2)

Publications using DYC2288-2

• D Laubitz, MA Gurney, M Midura-Kie, C Clutter, DG Besselsen, H Chen, FK Ghishan, PR Kiela Decreased NHE3 expression in colon cancer is associated with DNA damage, increased inflammation and tumor growth Scientific Reports, 2022-08-30;12(1):14725. 2022-08-30 [PMID: 36042372] (Human)
• P Wigner, K Zielinski, S Michlewska, P Danielska, A Marczak, EJ Ricci, R Santos-Oli, M Szwed Disturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles Scientific Reports, 2021-02-15;11(1):3849. 2021-02-15 [PMID: 33589697] (Human)

Bioinformatics

• Gene Symbol: H2AX