Human NT-ProANP Quantikine ELISA Kit

JOY6 human iPSCs were differentiated into beating cardiomyocytes. After 21 days of differentiation cardiomyocytes were treated with 5 nM of Endothelin-1 (Catalog # 1160) for 25.5 hours to induce cardiac hypertrophy. The level of secreted Pro-Atrial Natriuretic peptide (ANP), which is known to be elevated during cardiac hypertrophy, was assessed using the Human NT-ProANP Quantikine® ELISA Kit (Catalog # DANP00). As expected, detection of Pro-ANP was increased in Endothelin-1 treated cells.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human NT-ProANP Quantikine ELISA Kit Summary

• Background: The Quantikine^® Human NT-ProANP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human NT-ProANP in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human NT-ProANP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human NT-ProANP showed linear curves that were parallel to the sta...ndard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human NT-ProANP.
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• Specificity: Natural and recombinant human NT-ProANP.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human NT-ProANP Quantikine ELISA Kit

• ANF
• ANP
• ANPatriopeptin
• ATFB6
• atrial natriuretic factor
• Atrial Natriuretic Peptide
• Cardionatrin
• CDD-ANF
• natriuretic peptide A
• natriuretic peptide precursor A
• NPPA
• PND
• PNDcardionatrin
• prepronatriodilatin

Background

Atrial Natriuretic peptide (ANP) is synthesized as a 151 aa preproprotein. The proprotein contains cardiodilatin (aa 26 - 92) and atrial natriuretic factor (ANF; aa 124 - 151) which become active after processing by the serine protease corin. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. ANF circulates in the plasma, eliciting natriuretic, diuretic, vasorelaxant and antimitogenic effects directed toward reduction of body fluid and blood pressure regulation. ANF acts through the cGMP-coupled NP receptor A. Human ANP and ANF share 84% and 96% aa identity with mouse ANP and ANF, respectively.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Atrial Natriuretic Peptide/ANP (DANP00)(4)

Publications using DANP00

• Nieto-Roca, L;Camblor Blasco, A;Devesa, A;Gómez-Talavera, S;Balaguer-Germán, J;Lumpuy-Castillo, J;Pello, AM;Dhier, LM;Lapeña, G;Jiménez, LL;Lorenzo, Ó;Tuñón, J;Ibáñez, B;Aceña, Á; Serum Biomarkers and Their Association with Myocardial Function and Exercise Capacity in Cardiac Transthyretin Amyloidosis Journal of cardiovascular development and disease 2024-04-30 [PMID: 38786964] (Human)
• Cortés, M;Lorenzo, O;Lumpuy-Castillo, J;Martínez-Albaladejo, S;Taibo-Urquía, M;Pello, AM;Bollas, AJ;Orejas, M;Navas, MÁ;Macia, E;Martínez, ME;Rueda, A;Tuñón, J; Dapagliflozin Improved Cardiac Function and Structure in Diabetic Patients with Preserved Ejection Fraction: Results of a Single Centre, Observational Prospective Study Journal of clinical medicine 2023-10-23 [PMID: 37892836] (Human)
• CA Couch, LA Fowler, V Parcha, P Arora, BA Gower Associations of atrial natriuretic peptide with measures of insulin and adipose depots Physiological Reports, 2023-03-01;11(5):e15625. 2023-03-01 [PMID: 36905117] (Human)
• H Zhang, X Mo, Z Zhou, Z Zhu, X HuangFu, T Xu, A Wang, Z Guo, Y Zhang Associations among NPPA gene polymorphisms, serum ANP levels, and hypertension in the Chinese Han population J Hum Hypertens, 2019-07-24;0(0):. 2019-07-24 [PMID: 31341238] (Human)

FAQs for Atrial Natriuretic Peptide/ANP (DANP00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.