Human IL-20 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-20 Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-20 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IL-20 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-20 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-20 showed linear curves that were parallel to the standard curves obtaine...d using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IL-20.
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• Specificity: Natural and recombinant human IL-20
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL20

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-20 Quantikine ELISA Kit

• Cytokine Zcyto10
• IL10D
• IL20
• IL-20
• IL-20ZCYTO10four alpha helix cytokine
• interleukin 20
• interleukin-20
• MGC96907

Background

Mature human and mouse IL-20 is a 152 amino acid protein that is not likely glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 binds two heterodimeric receptor complexes, IL-20 R alpha/IL-20 R beta and IL-22 R/IL-20 R beta. IL-20 is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor-expressing cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-20 (DL200)(5)

Publications using DL200

• K Wolk, D Wilsmann-T, K Witte, TC Brembach, C Kromer, S Gerdes, K Ghoreschi, K Reich, R Mössner, R Sabat Interleukin-19 Levels Are Increased in Palmoplantar Pustulosis and Reduced following Apremilast Treatment International Journal of Molecular Sciences, 2023-01-09;24(2):. 2023-01-09 [PMID: 36674793] (Human)
• A Steinert, I Linas, B Kaya, M Ibrahim, A Schlitzer, P Hruz, K Radulovic, L Terraccian, AJ Macpherson, JH Niess The Stimulation of Macrophages with TLR Ligands Supports Increased IL-19 Expression in Inflammatory Bowel Disease Patients and in Colitis Models J. Immunol., 2017-09-01;0(0):. 2017-09-01 [PMID: 28864472] (Human)
• Regulation of IL-20 Expression by Estradiol through KMT2B-Mediated Epigenetic Modification PLoS ONE, 2016-11-02;11(11):e0166090. 2016-11-02 [PMID: 27806114] (Human)
• Manfè Valentina, Fleckner Jan, Nørby Lisby Peder, Zhang Bo, Dai Hongjie, Keller Pernille Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips BMC Biotechnology 2015-12-01 [PMID: 26268325] (Human)
• Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis. Eur. J. Immunol., 2009-12-01;39(12):3570-81. 2009-12-01 [PMID: 19830738] (Human)

FAQs for IL-20 (DL200). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL20