Human FAP QuicKit ELISA

• Reactivity: Hu
• Conjugate: HRP

Human FAP QuicKit ELISA Summary

• Background: The Quantikine QuicKit Human FAP Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human FAP levels in cell culture supernates, serum, and plasma. It contains recombinant human FAP and antibodies raised against the recombinant protein. Results obtained for naturally occurring human FAP showed linear curves that were parallel to the standard curves obtained using the ...recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural FAP.
  Show More
• Specificity: Natural and recombinant human FAP.
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FAP

Applications/Dilutions

• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human FAP QuicKit ELISA

• 170 kDa melanoma membrane-bound gelatinase
• DKFZp686G13158
• DPPIV
• EC 3.4.21.-
• FAP
• FAPA
• Fibroblast Activation Protein alpha
• fibroblast activation protein, alpha
• Integral membrane serine protease
• Seprase
• vibronectin

Background

FAP (fibroblast activation protein-alpha; also known as seprase) is a type II transmembrane serine protease that degrades gelatin, Type I collagen, and N-terminal prolines. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas, and granulation tissue, and may play roles in tumor invasion, tissue remodeling, and wound repair.

FAQs for Fibroblast Activation Protein alpha/FAP (QK3715). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FAP