Human Apolipoprotein B/ApoB Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Apolipoprotein B/ApoB Quantikine ELISA Kit Summary

• Background: The Quantikine Human Apolipoprotein B/ApoB Immunoassay is a 4.5 hour solid phase ELISA designed to measure ApoB levels in serum and plasma.
• Specificity: Natural human ApoB
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: APOB

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Apolipoprotein B/ApoB Quantikine ELISA Kit

• Apo B-100
• APOB
• ApoB-100
• ApoB-48
• apolipoprotein B (including Ag(x) antigen)
• Apolipoprotein B
• apolipoprotein B-100
• apolipoprotein B48
• FLDB
• LDLCQ4
• mutant Apo B 100

Background

The apolipoproteins are a structurally-unrelated group of proteins that have some association with the transport of lipids in blood. Apolipoproteins, plus phospholipids, cholesterol and triglycerides, form spherical particles with a lipid/hydrophobic center and a (apolipo)protein coat. The apolipoprotein coat promotes aqueous solubility and serves as a ligand for lipoprotein receptors. HDL may contain apolipoproteins A, C, D, E, J, L and M, while LDL contains apolipoproteins B and E.

ApoAI and ApoA2 are major protein components of serum high-density lipoprotein (HDL) and are produced by the liver and small intestine. They are involved in reverse cholesterol transport from tissues to the liver. Polymorphisms of ApoA2 are associated with disorders of cholesterol and fatty acid metabolism. Human ApoB (Apolipoprotein B-100) is a 550 kDa, secreted, palmitoylated glycoprotein that is part of LDL and VLDL particles. It is made by liver and is 4536 aa in length. It binds LDL to the ApoB/E receptor. ApoC activates lipoprotein lipase and may self-associate to form amyloid-type fibrils.

ApoE is a 34 kDa protein component of serum chylomicrons, VLDL, and HDL particles. It mediates the binding, uptake, and catabolism of these particles through interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is important in fatty acid homeostasis and memory formation. Polymorphisms encode three variants (ApoE2, 3, 4) which are differentially related to the development of atherosclerosis and neurogenerative disorders, particularly Alzheimer's disease.

Serum amyloid A proteins (SAAs) are a family of homologous apolipoproteins of high density lipoprotein (HDL). They can be divided into two groups. The first group consists of the acute phase SAA1 and SAA2 that associate with HDL during inflammation and remodel the HDL particle by displacing apolipoprotein A1. The second group consists of constitutively expressed SAA4 and SAA5 that exist as minor apolipoproteins on HDL but make up more than 90% of the total SAA during homeostasis.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Apolipoprotein B/ApoB (DAPB00)(7)

Publications using DAPB00

• PF Lebeau, JH Byun, K Platko, P Saliba, M Sguazzin, ME MacDonald, G Paré, GR Steinberg, LJ Janssen, SA Igdoura, MA Tarnopolsk, SR Wayne Chen, NG Seidah, J Magolan, RC Austin Caffeine blocks SREBP2-induced hepatic PCSK9 expression to enhance LDLR-mediated cholesterol clearance Nature Communications, 2022-02-09;13(1):770. 2022-02-09 [PMID: 35140212] (Human)
• MJ Koren, PM Moriarty, SJ Baum, J Neutel, M Hernandez-, HS Weintraub, M Florio, H Kassahun, S Melquist, T Varrieur, SM Haldar, W Sohn, H Wang, M Elliott-Da, BM Rock, T Pei, O Homann, J Hellawell, GF Watts Preclinical development and phase 1 trial of a novel siRNA targeting lipoprotein(a) Nature Medicine, 2022-01-13;0(0):. 2022-01-13 [PMID: 35027752] (Cynomolgus Monkey)
• SA Walker, JS Aguilar Dí, S Busatto, GA Wurtz, AC Zubair, CR Borges, J Wolfram Glycan Node Analysis of Plasma-Derived Extracellular Vesicles Cells, 2020-08-22;9(9):. 2020-08-22 [PMID: 32842648] (Human)
• P Jegatheesa, K Seyssel, N Stefanoni, V Rey, P Schneiter, V Giusti, V Lecoultre, L Tappy Effects of gastric bypass surgery on postprandial gut and systemic lipid handling Clin Nutr ESPEN, 2019-11-29;35(0):95-102. 2019-11-29 [PMID: 31987128] (Human)
• A Stachowicz, M Zabczyk, J Natorska, M Suski, R Olszanecki, R Korbut, JR Wi?niewski, A Undas Differences in plasma fibrin clot composition in patients with thrombotic antiphospholipid syndrome compared with venous thromboembolism Sci Rep, 2018-11-23;8(1):17301. 2018-11-23 [PMID: 30470809] (Human)
• D Grünig, A Felser, U Duthaler, J Bouitbir, S Krähenbühl Effect of the catechol-O-methyltransferase inhibitors tolcapone and entacapone on fatty acid metabolism in HepaRG cells Toxicol. Sci., 2018-08-01;0(0):. 2018-08-01 [PMID: 29688484] (Human)
• JW Wang, YN Zhang, SK Sze, SM van de Weg, F Vernooij, AH Schoneveld, SH Tan, HH Versteeg, L Timmers, CSP Lam, DPV de Kleijn Lowering Low-Density Lipoprotein Particles in Plasma Using Dextran Sulphate Co-Precipitates Procoagulant Extracellular Vesicles Int J Mol Sci, 2017-12-29;19(1):. 2017-12-29 [PMID: 29286309] (Human)

Bioinformatics

• Gene Symbol: APOB