HTF9C Antibody (OTI1C3) - Azide and BSA Free

Western Blot: HTF9C Antibody (OTI1C3) - Azide and BSA Free [NBP2-71830] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HTF9C.

Immunocytochemistry/Immunofluorescence: HTF9C Antibody (OTI1C3) - Azide and BSA Free [NBP2-71830] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY HTF9C.

Immunohistochemistry: HTF9C Antibody (OTI1C3) - Azide and BSA Free [NBP2-71830] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: HTF9C Antibody (OTI1C3) - Azide and BSA Free [NBP2-71830] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: HTF9C Antibody (OTI1C3) - Azide and BSA Free [NBP2-71830] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: HTF9C Antibody (OTI1C3) - Azide and BSA Free [NBP2-71830] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: HTF9C Antibody (OTI1C3) - Azide and BSA Free [NBP2-71830] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: HTF9C Antibody (OTI1C3) - Azide and BSA Free [NBP2-71830] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

CyTOF-ready: HTF9C Antibody (OTI1C3) - Azide and BSA Free [NBP2-71830] - Analysis of U2-OS cells.

CyTOF-ready: HTF9C Antibody (OTI1C3) - Azide and BSA Free [NBP2-71830] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by TRMT2A antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HTF9C Antibody (OTI1C3) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human TRMT2A(NP_073564) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRMT2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-2000
• Theoretical MW: 68.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HTF9C Antibody (OTI1C3) - Azide and BSA Free

• HTF9CHpaII tiny fragments locus 9c protein
• MGC102728
• TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae)
• tRNA (uracil-5-)-methyltransferase homolog A
• tRNA methyltransferase 2 homolog A

Background

HTF9C may be involved in nucleic acid metabolism and/or modifications

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRMT2A