Recombinant Human Hsp47 His Protein

SDS-Page: Recombinant Human Hsp47 Protein [NBC1-22576]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human Hsp47 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 18-418 of Human Hsp47
  
  

  Source: E.coli

  Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA EVKKPAAAAA PGTAEKLSPK AATLAERSAG LAFSLYQAMA KDQAVENILV SPVVVASSLG LVSLGGKATT ASQAKAVLSA EQLRDEEVHA GLGELLRSLS NSTARNVTWK LGSRLYGPSS VSFADDFVRS SKQHYNCEHS KINFRDKRSA LQSINEWAAQ TTDGKLPEVT KDVERTDGAL LVNAMFFKPH WDEKFHHKMV DNRGFMVTRS YTVGVMMMHR TGLYNYYDDE KEKLQIVEMP LAHKLSSLII LMPHHVEPLE RLEKLLTKEQ LKIWMGKMQK KAVAISLPKG VVEVTHDLQK HLAGLGLTEA IDKNKADLSR MSGKKDLYLA SVFHATAFEL DTDGNPFDQD IYGREELRSP KLFYADHPFI FLVRDTQSGS LLFIGRLVRP KGDKMRDEL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SERPINH1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 48.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Hsp47 His Protein

• (collagen binding protein 1)
• Arsenic-transactivated protein 3
• AsTP3
• BERF-1
• CBP1
• CBP2
• CBP2RA-A47
• Cell proliferation-inducing gene 14 protein
• Collagen-binding protein
• colligen
• Colligin
• colligin-1
• colligin-2
• gp46
• HSP47
• HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
• member 1, (collagen binding protein 1)
• member 2
• member 2, (collagen-binding protein 2)
• OI10
• PPROM
• proliferation-inducing gene 14
• RA-A47
• rheumatoid arthritis antigen A-47,47 kDa heat shock protein
• Rheumatoid arthritis-related antigen RA-A47
• serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
• Serpin H1
• serpin peptidase inhibitor, clade H (heat shock protein 47), member 1
• SERPINH1
• SERPINH2colligin

Background

Description: Heat shock protein 47(HSP47), also known as Colligin or Serpin H1, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein acts as a molecular chaperone facilitating the folding and assembly of procollagen molecules, retaining unfolded molecules within the ER, and assisting the transport of correctly folded-molecules from the ER to Golgi apparatus. Recombinant human HSP47/Colligin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SERPINH1