HSP27 Antibody (3G3)

Western Blot: HSP27 Antibody (3G3) [H00003315-M04] - Analysis of HSPB1 expression in transfected 293T cell line by HSPB1 monoclonal antibody (M04), clone 3G3. Lane 1: HSPB1 transfected lysatE (22.8 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: HSP27 Antibody (3G3) [H00003315-M04] - Analysis of monoclonal antibody to HSPB1 on HeLa cell. Antibody concentration 10 ug/ml

ELISA: HSP27 Antibody (3G3) [H00003315-M04] - Detection limit for recombinant GST tagged HSPB1 is approximately 0.1ng/ml as a capture antibody.

Immunoprecipitation: HSP27 Antibody (3G3) [H00003315-M04] - Analysis of HSPB1 transfected lysate using anti-HSPB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HSPB1 rabbit polyclonal antibody.

Proximity Ligation Assay: HSP27 Antibody (3G3) [H00003315-M04] - Analysis of protein-protein interactions between AKT1 and HSPB1. Mahlavu cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-HSPB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interactio

Product Discontinued

HSP27 Antibody (3G3) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HSPB1 (NP_001531, 96 a.a. ~ 205 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RVSLDVNHFAPDELTVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTQVSSSLSPEGTLTVEAPMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK
• Specificity: HSPB1 (3G3)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPB1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HSP27 Antibody (3G3)

• 28 kDa heat shock protein
• DKFZp586P1322
• Estrogen-regulated 24 kDa protein
• Heat shock 27 kDa protein
• heat shock 27kD protein 1
• heat shock 27kDa protein 1
• heat shock protein beta-1
• HMN2B
• HS.76067
• HSP25
• HSP27
• HSP27HSP 27
• HSP28CMT2F
• HSPB1
• SRP27
• Stress-responsive protein 27

Background

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPB1
• Entrez:
  • 3315(Human)
• OMIM:
  • 602195(Human)
• Uniprot:
  • NP_001531(Human)