HSP20/HSPB6 Antibody (SD086-03)

Western Blot: HSP20/HSPB6 Antibody (SD086-03) [NBP2-67815] - Analysis of HSP20/HSPB6 on rat skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: HSP20/HSPB6 Antibody (SD086-03) [NBP2-67815] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-HSP20/HSPB6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: HSP20/HSPB6 Antibody (SD086-03) [NBP2-67815] - Analysis of Hela cells with Hsp20 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Western Blot: HSP20/HSPB6 Antibody (SD086-03) [NBP2-67815] - Analysis of Hsp20 on different lysates using anti-Hsp20 antibody at 1/1,000 dilution. Positive control: Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle

Immunohistochemistry-Paraffin: HSP20/HSPB6 Antibody (SD086-03) [NBP2-67815] - Analysis of paraffin-embedded mouse heart tissue using anti-Hsp20 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HSP20/HSPB6 Antibody (SD086-03) [NBP2-67815] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Hsp20 antibody. Counter stained with hematoxylin.

Product Discontinued

HSP20/HSPB6 Antibody (SD086-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human HSP20/HSPB6 aa 81-130 / 160. (SwissProt: O14558 Human; SwissProt: Q5EBG6 Mouse; SwissProt: P97541 Rat)
• Localization: Cytoplasm, Secreted, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HSPB6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HSP20/HSPB6 Antibody (SD086-03)

• FLJ32389
• Heat shock 20 kDa-like protein p20
• heat shock protein beta-6
• heat shock protein, alpha-crystallin-related, B6
• HSP20
• HSPB6

Background

Hsp20 is a small heat shock protein related to Hsp25, Hsp27 and may form different hetercomplexes with these proteins. The specific physiological function of Hsp20 is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions, Hsp20 is diffusely distributed in the cytosol, but under heat stress conditions, it translocates to the nucleus. Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock. The Hsp20 was demonstrated to constitute up to 1.3% of the total cellular protein in vertebrate tissues, especially in muscle, and its expression is related to muscle contraction, specifically in slow-twitch muscle. Hsp20 may form different heterocomplexes with other Hsp's, such as alpha-crystalline and Hsp25. Phosphorylated form of Hsp20 is proposed to interact with monomeric actin whereas dephosphorylated form binds polymeric actin filaments. In normal conditions Hsp20 is diffusely disturbed in cytosol but under the heat stress it undergoes translocation to membrane fraction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPB6