HSF2 Antibody (3E2)

Western Blot: HSF2 Antibody (3E2) [NB110-96434] - Analysis (K562 cells) transiently transfected with control, HSF1 or HSF2 shRNA constructs using HSF1, HSF2 (3E2) antibodies. Courtesy of Lea Sistonen, Abo Akademi University, Finland.

Product Discontinued

HSF2 Antibody (3E2) Summary

• Immunogen: Purified recombinant mouse HSF2 protein
• Localization: Cytoplasm , Nucleus
• Specificity: Detects an approx. 69kDa protein in unstressed cells corresponding to HSF2 on SDS-PAGE immunoblots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: HSF2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Microarray
  • Western Blot 4 ug/ml
• Control:

  HSF2 Overexpression Lysate

Reactivity Notes

Human, Mouse, Rat, Guinea Pig, Hamster, Primate, Rabbit, Bovine, Sheep, Porcine, Canine

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for HSF2 Antibody (3E2)

• heat shock factor 2
• heat shock factor protein 2
• heat shock transcription factor 2MGC75048
• HSF 2
• HSF2
• HSTF 2
• HSTF2
• MGC117376
• MGC156196

Background

HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSF2
• Entrez:
  • 3298(Human)
  • 15500(Mouse)
  • 64441(Rat)
• Uniprot:
  • Q03933()