HSF2 Overexpression Lysate (Native)

Images

 
Western Blot: HSF2 Overexpression Lysate (Adult Normal) [NBL1-11737] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HSF2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HSF2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210751 and based on accession number NM_004506. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens heat shock transcription factor 2 (HSF2), transcript variant 1, mRNA.
Gene
HSF2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
60.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for HSF2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HSF2 Overexpression Lysate (Native)

  • heat shock factor 2
  • heat shock factor protein 2
  • heat shock transcription factor 2MGC75048
  • HSF 2
  • HSF2
  • HSTF 2
  • HSTF2
  • MGC117376
  • MGC156196

Background

HSF2, as well as the related gene HSF1, encodes a protein that binds specifically to the heat-shock element and has homology to HSFs of other species. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Although the names HSF1 and HSF2 were chosen for historical reasons, these peptides should be referred to as heat-shock transcription factors. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for HSF2 Lysate (NBL1-11737) (0)

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Bioinformatics

Gene Symbol HSF2
Entrez