HSF1 [p Ser326] Antibody (SU31-03)

Western Blot: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - Lane 1: Hela cells, whole cell lysate, 10ug/lane, Lane 2/3: Hela cells heated at 42 c for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Hela cells heated at 42 c for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-Phospho-HSF1(S326)antibody at 1:500 dilution.Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 57 kDa, Observed band size: 82 kDaBlocking and diluting buffer: 5% BSA.Exposure time: Lane 1/2 1 minute; Lane 3/4 2 minute 34 seconds.

Immunocytochemistry/Immunofluorescence: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - U937 human histiocytic lymphoma cell line stained with HSF1 [p Ser326] Antibody (SU31-03). ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HSF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - Analysis of Hela cells with p-HSF1(S326) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary

Western Blot: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - Analysis of p-HSF1(S326) on different lysates using anti-p-HSF1(S326) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: BT20 Lane 3: AGS

Immunocytochemistry/Immunofluorescence: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - Staining p-HSF1(S326) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - Staining p-HSF1(S326) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - Staining p-HSF1(S326) in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-p-HSF1(S326) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-HSF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-HSF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-HSF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-HSF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: HSF1 [p Ser326] Antibody (SU31-03) [NBP2-67455] - Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-HSF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

HSF1 [p Ser326] Antibody (SU31-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser326 of Human HSF1 aa 301-350 / 529. (SwissProt: Q00613 Human)
• Modification: p Ser326
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HSF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000
• Application Notes: HSF1 [p Ser326] antibody validated for ICC/IF from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HSF1 [p Ser326] Antibody (SU31-03)

• heat shock factor protein 1
• heat shock transcription factor 1HSTF1HSF 1
• HSF1
• HSTF 1
• HSTF1

Background

Human cells respond to heat stress by inducing the binding of a pre-existing transcriptional activator (heat shock factor, HSF) to DNA (1). Induction of heat shock protein (HSP) gene expression by stress is initiated by binding of HSF1 to HSP gene promoters to increase their transcription. The cytoprotective functions of these HSPs are essential for cell survival, and thus it is critical that inducible HSP gene expression be executed rapidly and efficiently. There is an interaction between heat shock factor 1 (HSF1) and symplekin, a protein known to form a complex with the polyadenylation factors CstF and CPSF. HSF1-symplekin complexes are detected only after stress treatment, and these two proteins co-localize in punctate nuclear structures in stressed cells (2). A chaperone/Hsp functioning as repressor of heat shock transcription factor (HSF) could make activation of hsp genes dependent on protein unfolding. It has been concluded that Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for HSF1 Antibody (NBP2-67455) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 08/30/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: U937 human histiocytic lymphoma cell line
• Species: Human
• Lot: SU31-03

Bioinformatics

• Gene Symbol: HSF1