Recombinant Human HRAS Protein

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SDS-Page: Recombinant Human HRAS Protein [NBP1-50831] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human HRAS Protein Summary

Description
A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-186 of Human vH RAS

Source: E.coli

Amino Acid Sequence: MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV REIRQHKLRK LNPPDESGPG CMSCKCLEHH HHHH

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
HRAS
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-50831.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Background

HRAS, also known as GTPase HRas, belongs to the Ras family which function in signal transduction pathways. This protein can bind GTP and GDP, and it has intrinsic GTPase activity. HRAS undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade. Defects in HRAS are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Recombinant human HRAS protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for HRAS Recombinant Protein (NBP1-50831)(1)

We have publications tested in 1 application: IA.


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Bioinformatics

Gene Symbol HRAS