HR6B/UBE2B Antibody (PCRP-UBE2B-1C7)

Immunocytochemistry/Immunofluorescence: HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [NBP3-07169] - Immunofluorescent Analysis of PFA-fixed HeLa cells. HR6B/UBE2B Mouse Monoclonal Antibody (PCRP-HR6B/UBE2B-1C7) followed by IgG-CF488 (green), counterstained with phalloidin.

Flow Cytometry: HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [NBP3-07169] - Flow Cytometric Analysis of PFA-fixed HeLa cells.HR6B/UBE2B Mouse Monoclonal Antibody (PCRP-HR6B/UBE2B-1C7) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Protein Array: HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [NBP3-07169] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HR6B/UBE2B Mouse Monoclonal Antibody (PCRP-HR6B/UBE2B-1C7).

• Reactivity: Hu
• Applications: Flow, ICC/IF, MA
• Clone: PCRP-UBE2B-1C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08912)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human HR6B/UBE2B protein (Uniprot: P63146)
• Localization: Cell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBE2B
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to work in Mouse, Rat, Xenopus and Zebrafish.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for HR6B/UBE2B Antibody (PCRP-UBE2B-1C7)

• E2 protein
• E2-17k
• E2-17kDa
• HHR6B
• HR6B
• RAD6 homolog B
• RAD6BEC 6.3.2.19
• UBC2
• UBE2B
• Ubiquitin carrier protein B
• ubiquitin-conjugating enzyme E2 B
• Ubiquitin-conjugating enzyme E2-17 kDa
• ubiquitin-conjugating enzyme E2B (RAD6 homolog)
• Ubiquitin-protein ligase B

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HR6B/UBE2B Antibody (NBP3-07169)(1)

Publication using NBP3-07169

• LB Volk, KL Cooper, T Jiang, ML Paffett, LG Hudson Impacts of arsenic on Rad18 and translesion synthesis Toxicology and Applied Pharmacology, 2022-09-08;454(0):116230. 2022-09-08 [PMID: 36087615]

Bioinformatics

• Gene Symbol: UBE2B
• Uniprot:
  • P63146(Human)