HP1 alpha Antibody (SR44-03)

Western Blot: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Analysis of HP1 alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: MCF-7 cell lysateLane 2: Hela cell lysate

Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Staining HP1 alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Analysis of paraffin-embedded rat uterus tissue with Rabbit anti-HP1 alpha antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Analysis of SH-SY-5Y cells with HP1 alpha antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Analysis of HP1 alpha on different cell lysates using anti-HP1 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela

Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Staining HP1 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Analysis of paraffin-embedded human tonsil tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Analysis of paraffin-embedded human uterus tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Analysis of paraffin-embedded mouse uterus tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Analysis of paraffin-embedded human tonsil tissue with Rabbit anti-HP1 alpha antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Analysis of paraffin-embedded human breast tissue with Rabbit anti-HP1 alpha antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

HP1 alpha Antibody (SR44-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human HP1 alpha aa 101-191 / 191. (SwissProt: P45973 Human; SwissProt: Q61686 Mouse; Entrez Gene: 300266 Rat)
• Localization: Nucleus, Chromosome
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CBX5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HP1 alpha Antibody (SR44-03)

• Antigen p25
• chromobox homolog 5 (Drosophila HP1 alpha)
• chromobox homolog 5 (HP1 alpha homolog, Drosophila)
• chromobox homolog 5
• chromobox protein homolog 5
• Heterochromatin protein 1 homolog alpha
• heterochromatin protein 1-alpha
• HP1 alpha homolog
• HP1 alpha
• HP1
• HP1A
• HP1-ALPHA
• HP1Hs alpha
• HP1Hs-alpha

Background

Heterochromatin protein-1 (HP1) is a methyl-lysine binding adapter protein that is involved in a variety important functions in the cell nucleus. Localized at heterochromatin sites, HP1 is responsible for gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.

HP1 antibodies are useful tools for research on transcription regulation and epigenetic signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CBX5