HOIP/RNF31 Antibody

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Immunohistochemistry: HOIP/RNF31 Antibody [NBP2-27290] - Analysis of RNF31 (HOIP) using RNF31 antibody. Human rectum probed with RNF31 antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Pm, Pm-Or, RMSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HOIP/RNF31 Antibody Summary

Immunogen
A portion of amino acids 950-1000 from human RNF31 (HOIP) was used as the immunogen for this antibody. NP-060469.4
Predicted Species
Chimpanzee (94%), Orangutan (94%), Rhesus Macaque (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNF31
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 94% homologous in Crab-eating Macaque, White-cheeked Gibbon, 88% in Guinea pig, Canine, common Rabbit, and Porcine, 82% in Chinese Hamster, gray short-tailed Opossum, Rat, and Xenopus.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for HOIP/RNF31 Antibody

  • EC 6.3.2.-
  • FLJ10111
  • FLJ23501
  • HOIP
  • MGC19975
  • ring finger protein 31
  • RNF31
  • ZIBRA
  • ZIBRAHOIL-1-interacting protein
  • Zinc in-between-RING-finger ubiquitin-associated domain protein

Background

RNF31, ring finger protein 31, is an E3 ubiquitin ligase also known as ZIBRA and HOIP is 110-120kDa. There are a number of functions attributed to RNF31 (HOIP) including zinc-ion binding and protein-poly ubiquitination (1-3). The NF-kB pathway is positively regulated by RNF31. T cell activation through T cell receptors is also modulated through RNF31. Based on its monoubiquitination of DAX-1, a mammalian orphan receptor associated with steroidogenesis, RNF31 (HOIP) also appears to be a requisite co-regulator of steroidogenic pathways (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RNF31