hnRNP M Antibody (3F7) - Azide and BSA Free

Western Blot: hnRNP M Antibody (3F7) [H00004670-M03] - Analysis of HNRPM expression in NIH/3T3 (Cat # L018V1).

Immunocytochemistry/Immunofluorescence: hnRNP M Antibody (3F7) [H00004670-M03] - Analysis of monoclonal antibody to HNRPM on HepG2 cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: hnRNP M Antibody (3F7) [H00004670-M03] - Analysis of monoclonal antibody to HNRPM on formalin-fixed paraffin-embedded human thyroid nodular goiter. Antibody concentration 3 ug/ml

Western Blot: hnRNP M Antibody (3F7) [H00004670-M03] - Analysis of HNRPM expression in PC-12 (Cat # L012V1).

Western Blot: hnRNP M Antibody (3F7) [H00004670-M03] - Analysis of HNRPM expression in HepG2 (Cat # L019V1).

ELISA: hnRNP M Antibody (3F7) [H00004670-M03] - Detection limit for recombinant GST tagged HNRPM is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

hnRNP M Antibody (3F7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HNRPM (NP_005959, 17 a.a. ~ 112 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSR
• Specificity: HNRPM - heterogeneous nuclear ribonucleoprotein M
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNRNPM
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for hnRNP M Antibody (3F7) - Azide and BSA Free

• DKFZp547H118
• heterogeneous nuclear ribonucleoprotein M
• hnRNP M
• HNRNPM4
• HNRPM4heterogenous nuclear ribonucleoprotein M
• HNRPMheterogenous nuclear ribonucleoprotein M4
• HTGR1
• M4 protein
• N-acetylglucosamine receptor 1
• NAGR1hnRNA-binding protein M4

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Multiple alternatively spliced transcript variants are known for this gene but only two transcripts has been isolated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for hnRNP M Antibody (H00004670-M03)(2)

Publications using H00004670-M03

• Yang T, An Z, Zhang C et al. hnRNPM, a potential mediator of YY1 in promoting the epithelial-mesenchymal transition of prostate cancer cells. Prostate. 2019-08-15 [PMID: 31251827]
• Jeon J, Jeong JH, Baek JH et al. Network Clustering Revealed the Systemic Alterations of Mitochondrial Protein Expression. PLoS Comput Biol 2011-06-30 [PMID: 21738461]

Bioinformatics

• Gene Symbol: HNRNPM
• Entrez:
  • 4670(Human)
• OMIM:
  • 160994(Human)
• Uniprot:
  • NP_005959(Human)