Recombinant Human hnRNP K His Protein

SDS-Page: hnRNP K Protein [NBP2-23120]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human hnRNP K His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-276 of Human hnRNP K
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMETEQPE ETFPNTETNG EFGKRPAEDM EEEQAFKRSR NTDEMVELRI LLQSKNAGAV IGKGGKNIKA LRTDYNASVS VPDSSGPERI LSISADIETI GEILKKIIPT LEEGLQLPSP TATSQLPLES DAVECLNYQH YKGSDFDCEL RLLIHQSLAG GIIGVKGAKI KELRENTQTT IKLFQECCPH STDRVVLIGG KPDRVVECIK IILDLISESP IKGRAQPYDP NFYDETYDYG GFTMMFDDRR GRPVGFPMRG RGGFDRMPPG RGGRPMPPS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: HNRNPK
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human hnRNP K His Protein

• dC-stretch binding protein
• FLJ41122
• heterogeneous nuclear ribonucleoprotein K
• hnRNP K
• transformation upregulated nuclear protein
• Transformation up-regulated nuclear protein
• TUNPHNRPKCSBP

Background

HNRNPK belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. HNRNPK is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. Recombinant human HNRNPK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for hnRNP K Protein (NBP2-23120)(1)

Publication using NBP2-23120

• Joseph Boissieras, Hugues Bonnet, Maria Fidelia Susanto, Dennis Gomez, Eric Defrancq, Anton Granzhan, Jérôme Dejeu iMab antibody binds single-stranded cytosine-rich sequences and unfolds DNA i-motifs. Nucleic acids research 2024-06-22 [PMID: 38908025]

Bioinformatics

• Gene Symbol: HNRNPK