hnRNP K/J Antibody (SC60-03)

Western Blot: hnRNP K/J Antibody (SC60-03) [NBP2-67581] - Analysis of hnRNP K on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: Hela cell lysate Lane 3: NIH/3T3 cell lysate

Immunocytochemistry/Immunofluorescence: hnRNP K/J Antibody (SC60-03) [NBP2-67581] - Staining hnRNP K in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: hnRNP K/J Antibody (SC60-03) [NBP2-67581] - Analysis of paraffin-embedded mouse brain tissue using anti-hnRNP K antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: hnRNP K/J Antibody (SC60-03) [NBP2-67581] - Staining hnRNP K in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: hnRNP K/J Antibody (SC60-03) [NBP2-67581] - Staining hnRNP K in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: hnRNP K/J Antibody (SC60-03) [NBP2-67581] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-hnRNP K antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: hnRNP K/J Antibody (SC60-03) [NBP2-67581] - Analysis of paraffin-embedded human colon cancer tissue using anti-hnRNP K antibody. Counter stained with hematoxylin.

Product Discontinued

hnRNP K/J Antibody (SC60-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human hnRNP K/J aa 1-49 / 463. (SwissProt: P61978 Human; SwissProt: P61979 Mouse; SwissProt: P61980 Rat)
• Localization: Nucleoplasm, Cytoplasm, podosome
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HNRNPK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for hnRNP K/J Antibody (SC60-03)

• dC-stretch binding protein
• FLJ41122
• heterogeneous nuclear ribonucleoprotein K
• hnRNP K
• transformation upregulated nuclear protein
• Transformation up-regulated nuclear protein
• TUNPHNRPKCSBP

Background

The hnRNP K / J gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). ThehnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associatedwith pre-mRNAs in the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPK