hnRNP G Antibody

Western Blot: hnRNP G Antibody [NBP2-34152] - Analysis in human cell line MOLT-4.

Immunocytochemistry/Immunofluorescence: hnRNP G Antibody [NBP2-34152] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: hnRNP G Antibody [NBP2-34152] - Staining of human skeletal muscle shows strong nuclear positivity in myocytes.

Immunohistochemistry-Paraffin: hnRNP G Antibody [NBP2-34152] - Staining of human duodenum shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: hnRNP G Antibody [NBP2-34152] - Staining of human endometrium shows strong nuclear positivity.

Immunohistochemistry-Paraffin: hnRNP G Antibody [NBP2-34152] - Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.

Product Discontinued

hnRNP G Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: GGHMDDGGYSMNFNMSSSRGP
• Predicted Species: Mouse (95%), Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBMX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for hnRNP G Antibody

• Glycoprotein p43
• heterogeneous nuclear ribonucleoprotein G
• hnRNP G
• hnRNP-G
• HNRPG
• RBMXP1
• RBMXRT
• RNA binding motif protein, X chromosome
• RNA binding motif protein, X-linked
• RNA-binding motif protein, X chromosome
• RNMX

Background

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. hnRNP G is a glycoprotein, which was originally identified as an autoantigen from German shepherd dogs with lupus-like syndrome. The gene encoding hnRNP G is located on chromosome Xq26 and is ubiquitously expressed. It contains one RNP-consensus RNA binding domain (RBD) and is related to RBMY, which is involved in spermatogenesis, and hnRNP G-T, which is a testis specific protein. All three proteins interact with Tra2b and therefore, are involved in pre-mRNA splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for hnRNP G Antibody (NBP2-34152)(1)

Publication using NBP2-34152

• Zhou KI, Shi H, Lyu R et al. Regulation of Co-transcriptional Pre-mRNA Splicing by m6A through the Low-Complexity Protein hnRNPG Mol. Cell 2019-07-30 [PMID: 31445886]

Bioinformatics

• Gene Symbol: RBMX
• Uniprot:
  • P38159(Human)