Recombinant Human HMGA1 His Protein

SDS-Page: Recombinant Human HMGA1 Protein [NBP1-72385]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human HMGA1 His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-107 of Human HMGA1
  
  

  Source: E.coli

  Amino Acid Sequence: MSESSSKSSQ PLASKQEKDG TEKRGRGRPR KQPPVSPGTA LVGSQKEPSE VPTPKRPRGR PKGSKNKGAA KTRKTTTTPG RKPRGRPKKL EKEEEEGISQ ESSEEEQLEH HHHHH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: HMGA1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 12.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 50% glycerol, 0.2 M NaCl
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human HMGA1 His Protein

• high mobility group AT-hook 1
• High mobility group AT-hook protein 1
• High mobility group protein A1
• high mobility group protein HMG-I/HMG-Y
• High mobility group protein R
• high-mobility group (nonhistone chromosomal) protein isoforms I and Y
• HMGA1A
• HMG-I(Y)
• HMGIYMGC12816
• HMG-R
• MGC4242
• MGC4854
• nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y

Background

High mobility group protein HMG-I/HMG-Y, also known as HMGA1, is a member of the non-histone chromosomal high mobility group protein (HMG) family. HMGA1 consists of a highly conserved AT-hook DNA-binding domain that mediates binding to AT-rich sequences in the minor groove of chromosomal DNA. It functions as architectural chromatin-binding transcription factor altering the conformation of DNA by modulating nuclear protein-DNA complexes. It is involved in many cellular processes including growth regulation, viral induction of beta-IFN gene and regulation of inducible gene transcription. Recombinant human HMGA1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HMGA1