HMGA1 Overexpression Lysate (Native)


Western Blot: HMGA1 Overexpression Lysate (Adult Normal) [NBL1-11612] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HMGA1.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

HMGA1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC201458 and based on accession number NM_002131. The protein contains a C-terminal DDK tag.
Homo sapiens high mobility group AT-hook 1 (HMGA1), transcript variant 2, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
10.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for HMGA1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HMGA1 Overexpression Lysate (Native)

  • high mobility group AT-hook 1
  • High mobility group AT-hook protein 1
  • High mobility group protein A1
  • high mobility group protein HMG-I/HMG-Y
  • High mobility group protein R
  • high-mobility group (nonhistone chromosomal) protein isoforms I and Y
  • HMGA1A
  • HMG-I(Y)
  • HMGIYMGC12816
  • HMG-R
  • MGC4242
  • MGC4854
  • nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y


This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) utilizes an alternate in-frame splice site in one of the coding exons compared to variant 1, resulting in a shorter isoform (b, also called HMG-Y) than isoform a (also called HMG-I). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for HMGA1 Lysate (NBL1-11612) (0)

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Gene Symbol HMGA1