HLA DRB1 Antibody (1091354) [Unconjugated]

HLA DRB1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human HLA DRB1 Monoclonal Antibody (Catalog # MAB11640) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 1091354
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HLA DRB1 Antibody (1091354) [Unconjugated] Summary

• Immunogen: Synthetic Peptide
  Accession # P01911
• Specificity: Detects a synthetic peptide specific for human HLA-DRB1 around amino acid 45 in Direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DRB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HLA DRB1 Antibody (1091354) [Unconjugated]

• Clone P2-beta-3
• DR1
• DR-1
• DR12
• DR-12
• DR13
• DR-13
• DR14
• DR-14
• DR16
• DR-16
• DR4
• DR-4
• DR5
• DR-5
• DR7
• DR-7
• DR8
• DR-8
• DR9
• DR-9
• DRB1
• DRw10
• DRw11
• DRw8
• DW2.2/DR2.2
• FLJ75017
• FLJ76359
• HLA class II antigen beta chain
• HLA class II histocompatibility antigen, DR-1 beta chain
• HLA-DR1B
• HLA-DRB
• HLA-DRB1
• HLA-DRB1*
• HLA-DRB2
• HLA-DR-beta 1
• human leucocyte antigen DRB1
• leucocyte antigen DR beta 1 chain
• leucocyte antigen DRB1
• lymphocyte antigen DRB1
• major histocompatibility complex, class II, DR beta 1
• MHC class II antigen DRB1*1
• MHC class II antigen DRB1*10
• MHC class II antigen DRB1*11
• MHC class II antigen DRB1*12
• MHC class II antigen DRB1*13
• MHC class II antigen DRB1*14
• MHC class II antigen DRB1*15
• MHC class II antigen DRB1*16
• MHC class II antigen DRB1*3
• MHC class II antigen DRB1*4
• MHC class II antigen DRB1*7
• MHC class II antigen DRB1*8
• MHC class II antigen DRB1*9
• MHC class II antigen HLA-DR13
• MHC class II HLA-DR beta 1 chain
• MHC class II HLA-DR-beta cell surface glycoprotein
• MHC class II HLA-DRw10-beta
• SS1

Background

HLA-DRB1 is an HLA class II histocompatibility antigen.  It is part of a heterodimer consisting of an alpha (DRA) and a beta chain (DRB) which are both anchored in the membrane.  Class II molecules are constitutively expressed in professional APC's. HLA-II is an important immune system protein that plays a role in tumor antigen presentation, immune response, and immune surveillance.  HLA-DRB1 presents antigenic peptides to HLA-DRB1-restricted CD4+ T-cells. Aberrant expression of HLA-II is closely related to the pathogenesis of tumors.

1. "Entrez Gene:  HLA-DRB1 major histocompatibility complex, Class II, DR beta I"
2. Hu JM, Li L, Chen YZ, Liu C, Cui X, Yin L, Yang L, Zou H, Pang L, Zhao J, Qi Y, Cao Y, Jiang J, Liang W, Li F. HLA-DRB1 and HLA-DQB1 methylation changes promote the occurrence and progression of Kazakh ESCC. Epigenetics. 2014 Oct;9(10):1366-73. doi: 10.4161/15592294.2014.969625. PMID: 25437052; PMCID: PMC4623353.
3. Zhao X, Li J, Zhu Q, Liang G, Xia W, He X, Zhu C, Qi H, Deng B, Chen X, Zhang J. HLA-A and HLA-DRB1 may play a unique role in ovarian teratoma-associated anti-N-methyl-D-aspartate receptor encephalitis. Reprod Biol Endocrinol. 2020 Nov 7;18(1):107. doi: 10.1186/s12958-020-00661-5. PMID: 33160385; PMCID: PMC7648266.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for HLA DRB1 Antibody (MAB11640). (Showing 1 - 1 of 1 FAQ).

1. We are interested in custom labeling our antibodies to be able to integrate new markers into already existing multicolor FACS panels. We would be glad to receive pricing and general order information for anti-HLA-DRB1 and anti-HLA-DRB5. To estimate expenses for future antibody labeling orders it would be helpful to know on what basis (amount, type, etc.) the price is going to be calculated. Right now the antibodies are still in mouse ascites. Would it be possible to integrate the elution of the antibodies from the ascites before labeling as part of the service agreement?
   • While we do not generally do this kind of custom purification unless it is for very large amounts of antibody, we do have a number of kits available that may be of use to you. Since an antibody that is still in ascites can not be labeled, it will need to be purified. A list of our kits available for this can be found at the following link: AbSelect. Once the antibodies are purified, they can be labeled with any number of different colors. A list of these kits for labeling can be found at the following link: Lightning-Link.

Bioinformatics

• Gene Symbol: HLA-DRB1
• Uniprot:
  • P01911(Human)