HLA DRA Antibody (1C11A5)

Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mAb against HEK293 (1) and HLA-DRA (AA: 26-254)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis of Hela cells using HLA-DRA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Flow Cytometry: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis of MCF-7 cells using HLA-DRA mouse mAb (green) and negative control (red).

Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mAb against human HLA-DRA (AA: 26-254) recombinant protein. (Expected MW is 51.5 kDa)

Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mouse mAb against Ramos (1), and Raji (2) cell lysate.

ELISA: HLA DRA Antibody (1C11A5) [NBP2-61863] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 1C11A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HLA DRA Antibody (1C11A5) Summary

• Immunogen: Purified recombinant fragment of human HLA-DRA (AA: 26-254) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DRA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
• Theoretical MW: 28.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for HLA DRA Antibody (1C11A5)

• FLJ51114
• histocompatibility antigen HLA-DR alpha
• HLA class II histocompatibility antigen, DR alpha chain
• HLA-DRA1
• major histocompatibility complex, class II, DR alpha
• MHC cell surface glycoprotein
• MHC class II antigen DRA
• MLRW

Background

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. ;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DRA