HLA DRA Antibody (169-1B5.2) [Allophycocyanin]

Flow Cytometry: HLA DRA Antibody (169-1B5.2) [Allophycocyanin] [NBP2-47722APC] - A surface stain was performed on human peripheral blood lymphocytes with HLA DRA (169-1B5.2) antibody NBP2-47722APC and a matched isotype control NBP2-27231APC. Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646.

Flow Cytometry: HLA DRA Antibody (169-1B5.2) [Allophycocyanin] [NBP2-47722APC] - Canine whole blood cells, image courtesy of customer.

• Reactivity: Hu, Bv, Fe
• Applications: Flow, ICC/IF, CyTOF-ready, ICC/IF
• Clone: 169-1B5.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

HLA DRA Antibody (169-1B5.2) [Allophycocyanin] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Human peripheral blood leukocytes (Uniprot: P01903)
• Localization: Cell surface
• Specificity: This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DRA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for HLA DRA Antibody (169-1B5.2) [Allophycocyanin]

• FLJ51114
• histocompatibility antigen HLA-DR alpha
• HLA class II histocompatibility antigen, DR alpha chain
• HLA-DRA1
• major histocompatibility complex, class II, DR alpha
• MHC cell surface glycoprotein
• MHC class II antigen DRA
• MLRW

Background

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for HLA DRA Antibody (NBP2-47722APC) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: A G Flow Canine 03/22/2017

Summary

• Application: Flow Cytometry
• Sample Tested: Whole blood cells
• Species: Canine
• Lot: 3122-2pabx160328032017-apc

Bioinformatics

• Gene Symbol: HLA-DRA