HLA DRA Antibody (169-1B5.2)

Flow Cytometry: HLA DRA Antibody (169-1B5.2) [NBP2-45043] - Flow Cytometric Analysis of PFA-fixed Raji cells. HLA DRA Antibody (169-1B5.2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Flow Cytometry: HLA DRA Antibody (169-1B5.2) [NBP2-45043] - Using the Allophycocyanin direct conjugate Canine whole blood cells, image courtesy of customer.

Flow Cytometry: HLA DRA Antibody (169-1B5.2) [NBP2-45043] - A surface stain was performed on human peripheral blood lymphocytes with HLA DRA (169-1B5.2) antibody NBP2-47722APC and a matched isotype control NBP2-27231APC. Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646. Image using the Allophycocyanin form of this antibody.

SDS-Page: HLA DRA Antibody (169-1B5.2) [NBP2-45043]

• Reactivity: Hu, Bv, Fe
• Applications: Flow, PAGE, ICC/IF
• Clone: 169-1B5.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

HLA DRA Antibody (169-1B5.2) Summary

• Immunogen: Human peripheral blood leukocytes
• Localization: Cell surface
• Specificity: This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DRA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • SDS-Page
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: Hu-chromosome location: 6p21.3
  Molecular weight of antigen: 36kDa (alpha chain) and 27kDa (beta chain)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for HLA DRA Antibody (169-1B5.2)

• FLJ51114
• histocompatibility antigen HLA-DR alpha
• HLA class II histocompatibility antigen, DR alpha chain
• HLA-DRA1
• major histocompatibility complex, class II, DR alpha
• MHC cell surface glycoprotein
• MHC class II antigen DRA
• MLRW

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DRA
• Uniprot:
  • P01903()