HLA DR/DP Antibody (CL10962) - Azide and BSA Free

Analysis in human lymph node and testis tissues using NBP3-44261 antibody. Corresponding HLA DR/DP RNA-seq data are presented for the same tissues.

Staining of human cerebral cortex (Alzheimer disease) shows strong cytoplasmic positivity in microglia.

Staining of human lymph node shows strong membranous positivity in germinal center cells.

Staining of human rectum shows strong cytoplasmic positivity in lymphoid cells.

Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

Analysis in human cell line Daudi.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL10962
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HLA DR/DP Antibody (CL10962) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a synthetic peptide of P01903, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DRA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for HLA DR/DP Antibody (CL10962) - Azide and BSA Free

• FLJ51114
• histocompatibility antigen HLA-DR alpha
• HLA class II histocompatibility antigen, DR alpha chain
• HLA-DRA1
• major histocompatibility complex, class II, DR alpha
• MHC cell surface glycoprotein
• MHC class II antigen DRA
• MLRW

Background

Human MHC class II antigens are transmembrane glycoproteins composed of an alpha (36 kDa) and a beta chain (27kDa) and are expressed primarily on antigen presenting cells. Human MHC class II genes are located in the HLA-D region that encodes at least 6 alpha and 10 beta chain genes. Three loci, DR, DQ and DP, encode the major products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells and therefore have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DRA