HLA DR/DP Antibody (Bra-14) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
• Clone: Bra-14
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

HLA DR/DP Antibody (Bra-14) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Human REH cells
• Localization: Cell surface
• Specificity: Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36kDa) and a chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DRA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for HLA DR/DP Antibody (Bra-14) [CoraFluor™ 1]

• FLJ51114
• histocompatibility antigen HLA-DR alpha
• HLA class II histocompatibility antigen, DR alpha chain
• HLA-DRA1
• major histocompatibility complex, class II, DR alpha
• MHC cell surface glycoprotein
• MHC class II antigen DRA
• MLRW

Background

Human MHC class II antigens are transmembrane glycoproteins composed of an alpha (36 kDa) and a beta chain (27kDa) and are expressed primarily on antigen presenting cells. Human MHC class II genes are located in the HLA-D region that encodes at least 6 alpha and 10 beta chain genes. Three loci, DR, DQ and DP, encode the major products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells and therefore have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DRA