HLA DQB1 Antibody - Azide and BSA Free

Western Blot: HLA DQB1 Antibody [H00003119-B01P] - Analysis of HLA-DQB1 expression in human pancreas.

Flow Cytometry: HLA DQB1 Antibody [H00003119-B01P] - Analysis of negative control 293 cells (Black) and HLA-DQB1 expressing 293 cells (Green) using HLA-DQB1 purified mouse polyclonal antibody.

Western Blot: HLA DQB1 Antibody [H00003119-B01P] - Analysis of HLA-DQB1 expression in transfected 293T cell line by HLA-DQB1 polyclonal antibody. Lane 1: HLA-DQB1 transfected lysate(28.71 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HLA DQB1 Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: HLA-DQB1 (AAH12106.1, 1 a.a. - 261 a.a.) full-length human protein. MSWKKALRIPGGLRVATVTLMLAMLSTPVAEGRDSPEDFVYQFKGMCYFTNGTERVRLVTRYIYNREEYARFDSDVGVYRAVTPLGPPAAEYWNSQKEVLERTRAELDTVCRHNYQLELRTTLQRRVEPTVTISPSRTEALNHHNLLVCSVTDFYPAQIKVRWFRNDQEETTGVVSTPLIRNGDWTFQILVMLEMTPQRGDVYTCHVEHPSLQNPIIVEWRAQSESAQSKMLSGIGGFVLGLIFLGLGLIIHHRSQKGLLH
• Specificity: HLA-DQB1 - major histocompatibility complex, class II, DQ beta 1,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: HLA-DQB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been use for FACS.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HLA DQB1 Antibody - Azide and BSA Free

• DC-1 alpha chain
• DC-alpha
• HLA class II histocompatibility antigen, DQ alpha 1 chain-like
• HLA-DCA
• MHC class II DQA1

Background

HLA-DQB1 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DQB1
• Entrez:
  • 3119(Human)
• Uniprot:
  • AAH12106.1(Human)