HLA DOA Antibody - BSA Free

Immunohistochemistry-Paraffin: HLA DOA Antibody [NBP1-91993] - Analysis in human tonsil and liver tissues using NBP1-91993 antibody. Corresponding HLA-DOA RNA-seq data are presented for the same tissues.

Western Blot: HLA DOA Antibody [NBP1-91993] - Analysis in control (vector only transfected HEK293T lysate) and HLA-DOA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: HLA DOA Antibody [NBP1-91993] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: HLA DOA Antibody [NBP1-91993] - Staining of human tonsil shows strong granular cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: HLA DOA Antibody [NBP1-91993] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

Immunohistochemistry-Paraffin: HLA DOA Antibody [NBP1-91993] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HLA DOA Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GDFARFDPQGGLAGIAAIKAHLDILVERSNRSRAINVPPRVTVLPKSRVE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HLA-DOA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  HLA DOA Protein (NBP1-91993PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HLA DOA Antibody - BSA Free

• HLA class II histocompatibility antigen, DO alpha chain
• HLA-D0-alpha
• HLA-DNAHLADZ
• HLA-DZA
• lymphocyte antigen
• major histocompatibility complex, class II, DN alpha
• major histocompatibility complex, class II, DO alpha
• MHC class II antigen DOA
• MHC DN-alpha
• MHC DZ alpha

Background

HLA DOA, also known as HLA class II histocompatibility antigen DO alpha chain, is a 250 amino acid protein that is 28 kDa, found in lysosomes in B cells and controls HLA-DM-mediated peptide loading on MHC class II molecules. Current research is being done on several diseases and disorders including vogt-koyanagi-harada disease, alopecia universalis, alopecia, systemic lupus erythematosus, graft versus host disease, lupus erythematosus, rheumatoid arthritis, toxoplasmosis, autoimmune thyroiditis, leishmaniasis, myocarditis, diabetes mellitus, arthritis influenza, immunodeficiency, asthma, tuberculosis, thyroiditis, interferon, and hepatitis b. Interactions with the HLA DOA protein have shown to involve ENSG00000204252 and HLA-DMA proteins in immune response antigen presentation by MHC class II, G-protein signaling N-RAS regulation pathway, immune response IL-22 signaling pathway, immune response NFAT in immune response, immune response ICOS pathway in T-helper cell, phagosome, cell adhesion molecules (CAMs), antigen processing and presentation, intestinal immune network for IgA production,and type I diabetes mellitus pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DOA