Histone H3 Antibody

Images

 
Western Blot: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - Histone H3/Citrulline (R2,R8,R17) at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - HL60 cells treated with DMSO and Calcimycin Green: (at 1:1000 Blue Hoechst)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Histone H3 Antibody Summary

Immunogen
Synthetic peptide derived from N terminal region of Human Histone H3, containing Citrulline at position 2;8;17
Specificity
The rabbit polyclonal reacts specifically with human Histone H3 Citrulline at either position 2 and or 8 and or 17. Not yet tested in other species, but the should react with a wide range of species due to sequence homology.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST3H3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5 000 - 1:50000
  • Chromatin Immunoprecipitation 1:10-1:500
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:2 000 - 1:20000
  • Immunohistochemistry-Paraffin 1:2 000 - 1:0 000
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-57135 in the following applications:

Publications
Read Publications using NB100-57135.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Antiserum
Preservative
No Preservative
Purity
Unpurified
Reconstitution Instructions
Please note the larger size(s) come in lyophilized form and the sample size will be in liquid formate. Reconstitute with 0.2 ml distilled water.

Alternate Names for Histone H3 Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Methylation of arginine residues within histone H3 has been linked to active transcription. However, the mechanism of demethylation of histones has remained elusive. Recently a process termed deimination has been described that converts histone arginine to citrulline and antagonizes arginine methylation (Cuthbert et al., 2004).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-81964
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-07993
Species: Hu
NBP2-52486
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC, IF
NBP2-07996
Species: Hu
NB500-171
Species: Hu, Mu, Rt, Xp, Ye
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
H00008349-M06
Species: Hu
Applications: ELISA, IHC-P
NBP1-58095
Species: Hu
Applications: WB
NBP2-61489
Species: Hu, Vb
Applications: WB, ELISA, ICC/IF
NBP1-90058
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-85351
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56346
Species: Hu, Mu
Applications: WB, ELISA, IHC-P
NBL1-11569
Species: Hu
NB21-2024
Species: Hu, Mu, Rt, Ma
Applications: WB, ChIP, DB, ICC/IF
H00008330-R01
Species: Hu
NBP2-16840
Species: Hu, Mu, Rt
Applications: WB, DB, EM, ICC/IF, IHC, IHC-Fr, IHC-P
H00008351-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC-P
NBL1-11560
Species: Hu
H00008360-R01
Species: Hu
NBL1-11570
Species: Hu
NBP2-10888
Species: Hu

Publications for Histone H3 Antibody (NB100-57135)(2)

Reviews for Histone H3 Antibody (NB100-57135) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Human and Mouse.
We have 2 reviews tested in 1 application: WB.

Reviews using NB100-57135:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Histone H3 NB100-57135
Enlarge
reviewed by:
Anonymous
WB Human and Mouse 11/25/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse hepatocytes and HepG2 cells
SpeciesHuman and Mouse
Lot16H8
Western Blot Histone H3 NB100-57135
Enlarge
reviewed by:
Anonymous
WB Human 08/06/2016
View

Summary

ApplicationWestern Blot
SpeciesHuman
LotPO number: E0520256
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB100-57135). (Showing 1 - 3 of 3 FAQs).

  1. Would you please advise us what samples you used to generate WB image with NB100-57135?
    • Our lab has just confirmed NB100-57135 has been validated in Western blot with HL60 cells treated with DMSO and calcimycin to induce citrullination.
  2. What is the source of histone material used for the western blot with NB100-57135?
    • NB100-57135 has been validated in western blot with HL60 cells treated with DMSO and calcimycin to induce citrullination.
  3. Can you provide NB100-57135 biotynilated?
    • Unfortunately, item NB100-57135 cannot be offered in a directly conjugated format as the base product is sold as unpurified antisera.

Secondary Antibodies

 

Isotype Controls

Additional Histone H3 Products

Bioinformatics Tool for Histone H3 Antibody (NB100-57135)

Discover related pathways, diseases and genes to Histone H3 Antibody (NB100-57135). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Research Areas for Histone H3 Antibody (NB100-57135)

Find related products by research area.

Blogs on Histone H3.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Anonymous
11/25/2017
Application: WB
Species: Human and Mouse

Anonymous
08/06/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol HIST3H3
Entrez