Histone H3 Antibody


Western Blot: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - Histone H3/Citrulline (R2,R8,R17) at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - HL60 cells treated with DMSO and Calcimycin Green: (at 1:1000 Blue Hoechst)

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Histone H3 Antibody Summary

Synthetic peptide derived from N terminal region of Human Histone H3, containing Citrulline at position 2;8;17
Citrulline 17, Citrulline 8, Citrulline 2
The rabbit polyclonal reacts specifically with human Histone H3 Citrulline at either position 2 and or 8 and or 17. Not yet tested in other species, but the should react with a wide range of species due to sequence homology.
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Whole antisera Rabbit antisera.
No Preservative
This product is unpurified. The exact concentration of antibody is not quantifiable.
Reconstitution Instructions
Please note the larger size(s) come in lyophilized form and the sample size will be in liquid formate. Reconstitute with 0.2 ml distilled water.


Reviewed Applications
Read 1 Review rated 4
NB100-57135 in the following application:

Read Publications using NB100-57135.

Alternate Names for Histone H3 Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • histone H3.3
  • MGC87782
  • MGC87783


Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Methylation of arginine residues within histone H3 has been linked to active transcription. However, the mechanism of demethylation of histones has remained elusive. Recently a process termed deimination has been described that converts histone arginine to citrulline and antagonizes arginine methylation (Cuthbert et al., 2004).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H3 Antibody (NB100-57135)(2)

Reviews for Histone H3 Antibody (NB100-57135) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-57135:
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Images Ratings Applications Species Date Details
Western Blot Histone H3 NB100-57135
reviewed by:
WB Human 08/06/2016


ApplicationWestern Blot
LotPO number: E0520256
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB100-57135). (Showing 1 - 3 of 3 FAQs).

  1. Would you please advise us what samples you used to generate WB image with NB100-57135?
    • Our lab has just confirmed NB100-57135 has been validated in Western blot with HL60 cells treated with DMSO and calcimycin to induce citrullination.
  2. What is the source of histone material used for the western blot with NB100-57135?
    • NB100-57135 has been validated in western blot with HL60 cells treated with DMSO and calcimycin to induce citrullination.
  3. Can you provide NB100-57135 biotynilated?
    • Unfortunately, item NB100-57135 cannot be offered in a directly conjugated format as the base product is sold as unpurified antisera.

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Research Areas for Histone H3 Antibody (NB100-57135)

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Blogs on Histone H3.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol HIST3H3

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