Histone H3 [Monomethyl Lys4] Antibody

Images

 
Immunohistochemistry-Paraffin: Histone H3 [Monomethyl Lys4] Antibody [NB100-2209] - Immunoperoxidase staining of Anti-Methyl-Histone H3 [Me-Lys4] in formalin fixed, paraffin-embedded rat stomach at 0.1 ug/mL.
Immunohistochemistry-Paraffin: Histone H3 [Monomethyl Lys4] Antibody [NB100-2209] - Immunoperoxidase staining of Anti-Methyl-Histone H3 [Me-Lys4] in formalin fixed, paraffin-embedded mouse brain at 0.1 ug/mL.
Immunohistochemistry-Paraffin: Histone H3 [Monomethyl Lys4] Antibody [NB100-2209] - Immunoperoxidase staining of Anti-Methyl-Histone H3 [Me-Lys4] in formalin fixed, paraffin-embedded mouse testis at 0.1 ug/mL.
Immunohistochemistry-Paraffin: Histone H3 [Monomethyl Lys4] Antibody [NB100-2209] - Immunoperoxidase staining of Anti-Methyl-Histone H3 [Me-Lys4] in formalin fixed, paraffin-embedded rat pancreas at 0.1 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, DrSpecies Glossary
Applications WB, ChIP, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Histone H3 [Monomethyl Lys4] Antibody Summary

Immunogen
Synthetic methylated peptide corresponding to amino acid 1-8 (Me-Lys4) at the N-terminus of human histone H3, conjugated to KLH. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, C. elegans, tetrahymena, and Arabidopsis thaliana histone H3.
Modification
Monomethyl Lys4
Specificity
Detects a band at approximately 17kDa. Partial or no inhibition is observed with the di-methylated histone H3 [diMe-Lys4 ] peptide (human, amino acids 1-8) and the non-methylated histone H3 peptide (human, amino acids 1-8).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST3H3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Jurkat Lysate (NB800-PC2)
HeLa Lysate (NB820-59462)

Reactivity Notes

Cross-reacts with Human, Drosophila, Mouse, Bovine and Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone H3 [Monomethyl Lys4] Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • H3K4Me1
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783

Background

The relatively unstructured and highly charged N-terminal tail domains of histones are central to the processes that modulate chromatin structure. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, and methylation occur on the N-terminal tail domains of histones, particularly of H3 and H4.1,2 These modifications may alter chromatin structure and recruit downstream chromatin-associated proteins involved in transcription regulation. These in turn, may dictate dynamic transitions between transcriptionally active or silent chromatin states. Histones H3 and H4 are the predominant histones modified by methylation and are highly methylated in mammalian cells.3,4 Histone methylation like acetylation is a complex dynamic process involved in a number of processes including transcriptional regulation, chromatin condensation, mitosis, and heterochromatin assembly. Moreover, lysine residues can be mono-, di-, and trimethylated at different heterochromatic subdomains adding further complexity to the regulation of chromatin structure. Conserved lysines residues in the N-terminal tail domains of histone H3 (Lys4, Lys9, and Lys27) are the preferred sites of methylation.1,4-6 Histone H3 mono-, di-, and trimethylation at Lys4 and Lys9 are carried out both in vitro and in vivo by SET domain-, site-specific histone methyltransferases (HMTases), including Suv39h1, Suv39h2, and G9a.7,8 Di- and trimethylation of histone H3 at Lys4 in coding regions correlates with transcriptional activity of many genes.9,10 Dimethylation of Histone H3 at Lys4 occurs at both active and inactive euchromatic regions but not in silent heterochromatic sites, whereas trimethylation at Lys4 is present exclusively at active genes. Mono- and dimethylation of H3 at Lys9 are intrinsically linked to epigenetic silencing and heterochromatin assembly. In contrast, trimethylated H3 at Lys9 is enriched at pericentric heterochromatin domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Histone H3 Antibody (NB100-2209)

Discover related pathways, diseases and genes to Histone H3 Antibody (NB100-2209). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Histone H3.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Bioinformatics

Gene Symbol HIST3H3
Entrez
Uniprot