Histone H2AX Antibody (3F4) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody (3F4) [H00003014-M05] - Analysis of monoclonal antibody to H2AFX on HeLa cell. Antibody concentration 10 ug/ml

• Reactivity: Hu
• Applications: ELISA, ICC/IF
• Clone: 3F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Histone H2AX Antibody (3F4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: H2AFX (AAH04915, 1 a.a. ~ 143 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGRGKTGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGHYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTSATVGPKAPSGGKKATQASQEY
• Specificity: H2AFX (3F4)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: H2AX
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Antibody reactivity against recombinant protein on ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Histone H2AX Antibody (3F4) - Azide and BSA Free

• H2A.X
• H2A/X
• H2AFX
• Histone H2AX

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Histone H2AX Antibody (H00003014-M05) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ImageStream Human 10/10/2016

Summary

• Application: Other
• Sample Tested: WI-38 human lung fibroblast cell line
• Species: Human
• Lot: E7181-3F4

Bioinformatics

• Gene Symbol: H2AX
• Entrez:
  • 3014(Human)
• OMIM:
  • 601772(Human)
• Uniprot:
  • AAH04915(Human)