Histone Deacetylase 8/HDAC8 Antibody [Alexa Fluor® 750]

• Reactivity: Hu, Mu
• Applications: WB, ChIP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

Histone Deacetylase 8/HDAC8 Antibody [Alexa Fluor® 750] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HDAC8 isoform 1
  Met1-Val377 (Leu31Pro)
  Accession # Q9BY41
• Specificity: Detects human HDAC8 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Histone Deacetylase 8/HDAC8 Antibody [Alexa Fluor® 750]

• EC 3.5.1.98
• HD8
• HDAC8
• HDACL1
• Histone Deacetylase 8
• histone deacetylase-like 1
• RPD3

Background

HDAC8 (Histone deacetylase 8) is a 50 kDa member of the class I subfamily, histone deacetylase family of transcriptional regulators. It is a smooth muscle enzyme that deacetylates histone lysines, blocking gene transcription. HDAC8 appears to function as a monomer, and phosphorylation at Ser39 inhibits activity. Human HDAC8 is 377 amino acids (aa) in length. It contains a series of  alpha helices and  strands, plus a catalytic ferrous ion binding site (Asp178-His180-Asp267). There are four potential splice variants, all of which possess an alternate start site 38 aa upstream of the standard start site. Three have additional changes; two show a one and three aa substitution respectively for aa 211-377, while a third has a 15 aa substitution for aa 148-377. Human HDAC8 shares more than 96% aa identity with both mouse and canine HDAC8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.