Histone Deacetylase 2/HDAC2 Antibody (C.15200201) - BSA Free

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - Whole cell extracts from HeLa cells (25 ug) were analyzed using the antibody against HDAC2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Chromatin Immunoprecipitation: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - ChIP assays were performed using human HeLa cells, the antibody consisting of 1, 2 and 5 ug per ChIP experiment was analyzed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the ACTB, EIF4A2 and LMO4 genes, used as positive controls, and for the Sat2 satellite repeat, used as negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - Whole cell extracts (40 ug) from HeLa cells transfected with HDAC2 siRNA (lane 2) and from an untransfected control (lane 1) were analyzed using the antibody against HDAC2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.

• Reactivity: Hu
• Applications: WB, ICC/IF, ChIP, ICC/IF, Mycoplasma
• Clone: C.15200201
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Histone Deacetylase 2/HDAC2 Antibody (C.15200201) - BSA Free Summary

• Description: Novus Biologicals Mouse Histone Deacetylase 2/HDAC2 Antibody (C.15200201) - BSA Free (NBP2-59269) is a monoclonal antibody validated for use in WB, ICC/IF and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The exact immunogen is propietary information.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HDAC2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1 ug
  • Chromatin Immunoprecipitation Sequencing 1ug/ChIP
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunofluorescence 1:500
  • Knockdown Validated
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for Histone Deacetylase 2/HDAC2 Antibody (C.15200201) - BSA Free

• EC 3.5.1.98
• HD2
• HDAC2
• Histone Deacetylase 2
• RPD3
• transcriptional regulator homolog RPD3
• YAF1
• YY1-associated factor 1

Background

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC2