Histidase Antibody (4F2)

Western Blot: Histidase Antibody (4F2) [H00003034-M04] - Analysis of HAL expression in transfected 293T cell line by HAL monoclonal antibody (M04), clone 4F2. Lane 1: HAL transfected lysatE (72.698 KDa). Lane 2: Non-transfected lysate.

Immunoprecipitation: Histidase Antibody (4F2) [H00003034-M04] - Analysis of HAL transfected lysate using anti-HAL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HAL rabbit polyclonal antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
• Clone: 4F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Histidase Antibody (4F2) Summary

• Immunogen: HAL (NP_002099, 558 a.a. ~ 657 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LAACQGIEFLRPLKTTTPLEKVYDLVRSVVRPWIKDRFMAPDIEAAHRLLLEQKVWEVAAPYIEKYRMEHIPESRPLSPTAFSLQFLHKKSTKIPESEDL
• Specificity: HAL (4F2)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HAL
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Histidase Antibody (4F2)

• EC 4.3.1
• EC 4.3.1.3
• HIS
• histidase
• histidine ammonia-lyase
• HSTD

Background

Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histidase Antibody (H00003034-M04)(3)

Publications using H00003034-M04

• Zhu H, Wang N, Yao L et al. Moderate UV Exposure Enhances Learning and Memory by Promoting a Novel Glutamate Biosynthetic Pathway in the Brain. Cell 2018-05-17 [PMID: 29779945]
• Barresi C, Stremnitzer C, Mlitz V et al. Increased Sensitivity of Histidinemic Mice to UVB Radiation Suggests a Crucial Role of Endogenous Urocanic Acid in Photoprotection. J Invest Dermatol. 2010-08-05 [PMID: 20686493]
• Eckhart L, Schmidt M, Mildner M et al. Histidase expression in human epidermal keratinocytes: Regulation by differentiation status and all-trans retinoic acid. J Dermatol Sci. 2008-02-14 [PMID: 18280705]

Bioinformatics

• Gene Symbol: HAL
• Entrez:
  • 3034(Human)
• OMIM:
  • 235800(Human)
• Uniprot:
  • NP_002099(Human)