Histamine H3R Antibody (1008944) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) Histamine H3 R or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human Histamine H3 R Monoclonal Antibody (Catalog # MAB10200) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 1008944
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Histamine H3R Antibody (1008944) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human Histamine H3 R
  Accession # Q9Y5N1
• Specificity: Detects human Histamine H3 R in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Histamine H3R Antibody (1008944) [Unconjugated]

• GPCR97
• GPCR97G protein-coupled receptor 97
• G-protein coupled receptor 97
• H3R
• HH3R
• Histamine H3 R
• histamine H3 receptor
• Histamine H3R
• histamine receptor H3
• HRH3

Background

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors Histamine H1 R, Histamine H2 R, Histamine H3 R and Histamine H4 R. HRH3 encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. Histamine H3 R is an integral membrane protein and can regulate neurotransmitter release. It can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9Y5N1()