Histamine H1R Antibody

Western Blot: Histamine H1 R Antibody [NBP1-06039] - (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Histamine H1 R Antibody [NBP1-06039] - (4ug/ml) staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Histamine H1R Antibody Summary

• Description: Novus Biologicals Goat Histamine H1R Antibody (NBP1-06039) is a polyclonal antibody validated for use in IHC, WB, ELISA and Simple Western. Anti-Histamine H1R Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence QQTSVRREDKCETD corresponding to internal region according to NP_000852.1.
• Epitope: QQTSVRREDKCETD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HRH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2-4ug/ml
  • Immunohistochemistry-Paraffin 2-4ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Simple Western
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in human heart lysates (calculated MW of 55.8 kDa band according to NP_000852.1).
  HC: In paraffin embedded Human Lung shows membranous staining of epitheilial cells in tertiar bronchus and in alveoli.
  See Simple Western Antibody Database for Simple Western validation
• Control:

  Histamine H1R Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Histamine H1R Antibody

• H1R
• H1-R
• hisH1
• Histamine H1 R
• Histamine H1R
• histamine receptor H1
• HRH1
• subclass H1

Background

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histamine H1R Antibody (NBP1-06039)(2)

Publications using NBP1-06039

• Wicher Sa, Roos Bb, Teske Jj Et Al. Aging increases senescence, calcium signaling, and extracellular matrix deposition in human airway smooth muscle PloS one 2021-07-29 [PMID: 34324543] (WB, Simple Western)
• Bryce PJ, Mathias CB, Harrison KL et al. The H1 histamine receptor regulates allergic lung responses. J Clin Invest 2006-06-01 [PMID: 16680192]

Bioinformatics

• Gene Symbol: HRH1
• Entrez:
  • 3269(Human)
• Uniprot:
  • NP_000852.1(Human)