![Immunocytochemistry/Immunofluorescence: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HIC2 Antibody (PCRP-HIC2-1B1) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).](http://images.novusbio.com/fullsize/HIC2-Antibody-PCRP-HIC2-1B1-Immunocytochemistry-Immunofluorescence-NBP3-13890-img0002.jpg "Immunocytochemistry/Immunofluorescence: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HIC2 Antibody (PCRP-HIC2-1B1) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IP, MA |
| Clone | PCRP-HIC2-1B1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14231) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant full-length human HIC2protein (Uniprot: Q96JB3) |
| Localization | Nucleus |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HIC2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: For coating, order antibody without BSA Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate) |
| Theoretical MW | 66.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890] - Flow cytometric analysis of PFA-fixed HeLa cells. HIC2 antibody (PCRP-HIC2-1B1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/HIC2-Antibody-PCRP-HIC2-1B1-Flow-Cytometry-NBP3-13890-img0001.jpg "Flow Cytometry: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890] - Flow cytometric analysis of PFA-fixed HeLa cells. HIC2 antibody (PCRP-HIC2-1B1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).")
![Protein Array: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HIC2 Antibody (PCRP-HIC2-1B1).](http://images.novusbio.com/fullsize/HIC2-Antibody-PCRP-HIC2-1B1-Protein-Array-NBP3-13890-img0004.jpg "Protein Array: HIC2 Antibody (PCRP-HIC2-1B1) [NBP3-13890] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HIC2 Antibody (PCRP-HIC2-1B1).")
