hHpr1-p84-Thoc1 Antibody

Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - Detection of Human THOC1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THOC1 antibody NBP1-47304 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- THOC1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate

Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - Detection of human THOC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-THOC1 antibody NBP1-47304 used for IP at 3 ug per reaction. THOC1 was also immunoprecipitated by another rabbit anti-THOC1 antibody. For blotting immunoprecipitated THOC1, NBP1-47304 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

hHpr1-p84-Thoc1 Antibody Summary

• Description: Novus Biologicals Rabbit hHpr1-p84-Thoc1 Antibody (NBP1-47304) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 607 and 657 of human THO Complex 1 using the numbering given in entry NP_005122.2 (GeneID 9984).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: THOC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for hHpr1-p84-Thoc1 Antibody

• HPR1THO complex subunit 1
• hTREX84
• Nuclear matrix protein p84
• P84
• P84N5
• THO complex 1
• tho1

Background

P84/N5-5E10 recognizes an 84kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THOC1
• Entrez:
  • 9984(Human)